A first-in-human phase 1 study of ACE910, a novel factor VIII–mimetic bispecific antibody, in healthy subjects by Naoki Uchida, Takehiko Sambe, Koichiro Yoneyama, Naoki Fukazawa, Takehiko Kawanishi, Shinichi Kobayashi, and Midori Shima Blood Volume 127(13):1633-1641 March 31, 2016 ©2016 by American Society of Hematology

Study design and stepwise dose-escalation scheme. Study design and stepwise dose-escalation scheme. Stepwise dose-escalation scheme for healthy male Japanese subjects (part A) and white subjects (part B) is shown. A total of 40 Japanese subjects were recruited to part A, and a total of 24 white subjects were recruited to part B. Part A: Japanese subjects were randomized to receive a single subcutaneous dose of ACE910 (n = 6 for each dose group) or placebo (n = 2 for each dose group). The Japanese subjects were observed for 4 weeks at each dose, and data were reviewed prior to dose escalation. Part B: white subjects were randomized to receive a single subcutaneous injection of ACE910 (n = 6 for each dose group) or placebo (n = 2 for each dose group). The white subjects were observed for 4 weeks at each dose, and data were reviewed prior to dose escalation. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

Plasma ACE910 concentration after single subcutaneous injection of ACE910. Plasma ACE910 concentration after single subcutaneous injection of ACE910. The time courses of plasma ACE910 concentration after single subcutaneous injection of 0.01 mg/kg (pink reverse triangle), 0.1 mg/kg (green square), 0.3 mg/kg (blue triangle), and 1 mg/kg (red circle) of ACE910 in Japanese (A) and white (B) healthy subjects are shown. Plasma ACE910 concentration was below the lower limit of quantification in all subjects at a dose of 0.001 mg/kg. Results are presented as mean ± standard deviation. Data out of the quantification range were handled as missing in summary statistics calculation. Summary statistics were not calculated when a plasma ACE910 concentration was below the lower limit of quantification in the majority of subjects at a certain dose group and time point. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

Plasma FIX and FX concentrations after single subcutaneous injection of ACE910. Plasma FIX and FX concentrations after single subcutaneous injection of ACE910. The time courses of plasma FIX (A) and FX (B) concentration after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond), 0.01 mg/kg (pink reverse triangle), 0.1 mg/kg (green square), 0.3 mg/kg (blue triangle), and 1 mg/kg (red circle) of ACE910 in Japanese healthy subjects are shown. The concentrations of FIX and FX including their activated forms in plasma were determined. Results are presented as mean ± standard deviation. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

PD responses after single subcutaneous injection of ACE910 without neutralization of the endogenous FVIII. The time courses of APTT (A) and peak height of TG (B) after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond)... PD responses after single subcutaneous injection of ACE910 without neutralization of the endogenous FVIII. The time courses of APTT (A) and peak height of TG (B) after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond), 0.01 mg/kg (pink reverse-triangle), 0.1 mg/kg (green square), 0.3 mg/kg (blue triangle), and 1 mg/kg (red circle) of ACE910 in Japanese healthy subjects measured without neutralization of endogenous FVIII are shown. Results are presented as mean ± standard deviation. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

PD responses after single subcutaneous injection of ACE910 with neutralization of the endogenous FVIII. The time courses of APTT (A-B) and peak height of TG (C-D) after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond... PD responses after single subcutaneous injection of ACE910 with neutralization of the endogenous FVIII. The time courses of APTT (A-B) and peak height of TG (C-D) after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond), 0.01 mg/kg (pink reverse triangle), 0.1 mg/kg (green square), 0.3 mg/kg (blue triangle), and 1 mg/kg (red circle) of ACE910 in Japanese (A,C) and white (B,D) healthy subjects measured with neutralization of endogenous FVIII are shown. TG was undetectable at baseline in the majority of subjects in each dose group. TG remained undetectable throughout the study period in the majority of subjects receiving placebo (data not shown). Results are presented as mean ± standard deviation. Data out of the quantification range were handled as missing in summary statistics calculation. Summary statistics were not calculated when TG was undetectable in the majority of subjects at a certain dose group and time point. One observed data point of TG was excluded because of being judged as an outlier, considering its unlikely time course and drug concentration dependency. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

Coagulation markers and platelet count after single subcutaneous injection of ACE910. Coagulation markers and platelet count after single subcutaneous injection of ACE910. The time courses of d-dimer (A), TAT (B), PT-INR (C), and platelet count (D) after single subcutaneous injection of placebo (black circle), 0.001 mg/kg (light blue diamond), 0.01 mg/kg (pink reverse triangle), 0.1 mg/kg (green square), 0.3 mg/kg (blue triangle), and 1 mg/kg (red circle) of ACE910 in Japanese healthy subjects are shown. Results are presented as mean ± standard deviation. The data out of the quantification range were imputed by the limit of quantification values. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology

Effect of anti-ACE910 antibodies on PK and PD of ACE910. Effect of anti-ACE910 antibodies on PK and PD of ACE910. The PK and PD profiles in ADA-positive subject (red open square) and ADA-negative subjects (black open circle) are shown. (A) Plasma ACE910 concentration in the Japanese 0.1 mg/kg group. (B) APTT in the Japanese 0.1 mg/kg group. (C) Peak height of TG in the Japanese 0.1 mg/kg group. (D) Plasma ACE910 concentration in the white 0.1 mg/kg group. (E) APTT in the white 0.1 mg/kg group. (F) Peak height of TG in the white 0.1 mg/kg group. The data out of the quantification range were imputed by the limit of quantification values. One observed data point of TG was excluded because of being judged as an outlier, considering its unlikely time course and drug concentration dependency. Naoki Uchida et al. Blood 2016;127:1633-1641 ©2016 by American Society of Hematology