CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

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Neal et al.Supplementary Figure S1

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CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. A, siRNA-transfected MDA-MB-231 cells were stained with immunofluorescence by using biotinylated CXCR4 antagonistic peptide and streptavidin-phycoerythrin. Red, CXCR4 phycoerythrin staining; blue, counterstaining of nuclei. B, RT-PCR analysis of CXCR4 of the siRNA-transfected MDA-MB-231 cells showed that siRNA1+2 effectively blocked the expression of CXCR4 mRNA. Western blot results of the siRNA-transfected MDA-MB-231 cells by using anti-CXCR4 antibody Ab-2 (1:1,000) showed that siRNA1+2 blocked the expression of CXCR4 protein almost completely. β-Actin (Sigma, 1:2,500) was used as a loading control. Cont, control. Zhongxing Liang et al. Cancer Res 2005;65:967-971 ©2005 by American Association for Cancer Research