Ectopic expression of miR-187 in MCF7 cells results in increased migration, invasion, and anchorage-independent colony formation. Ectopic expression of.

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Ectopic expression of miR-187 in MCF7 cells results in increased migration, invasion, and anchorage-independent colony formation. Ectopic expression of miR-187 in MCF7 cells results in increased migration, invasion, and anchorage-independent colony formation. A and B, MTT cell viability assay showing response of miR-187 expressing MCF7 cells to antiestrogens. Graph is representative of 1 of 3 independent repeats (± SD). No statistically significant change in response to either drug was noted (P = 0.407 for tamoxifen and P = 0.425 for fulvestrant). C, scratch wound assay showing that MCF7 cells expressing miR-187 display greater migratory potential than parental MCF7 cells or those infected with a NTC vector for 1 of 3 independent repeats ± SD; P = 0.005 relative to NTC cells, Student t test based on the slope of each line. D, soft-agar colony formation assay showing that expression of miR-187 in MCF7 cells increases anchorage-independent growth compared with control cell lines for 1 of 3 independent repeats, ±SD. E, spheroid invasion assay showing that spheroids formed by MCF7 cells expressing miR-187 show increased invasion into collagen as compared with control cell lines. F, Western blot analysis of MMP13 in cell lysates with β-actin as a loading control. Laoighse Mulrane et al. Clin Cancer Res 2012;18:6702-6713 ©2012 by American Association for Cancer Research