Identification algorithm combining the use of commercial DNA probes (rounded rectangles and squares) and genetic sequencing (ellipses). Identification.

Slides:

Advertisements

Similar presentations

MYCOBACTERIOLOGY 2004 What laboratories are doing and where they are headed Current identification techniques Molecular Current identification techniques.

Advertisements

Patterns. PATTERNS A pattern constitutes a set of numbers or objects in which all the members are related with each other by a specific rule. It is also.

Chapter 20 DNA Technology and Genomics. Biotechnology is the manipulation of organisms or their components to make useful products. Recombinant DNA is.

T. Trimpe One of SpongeBob’s cousins with hybrid round eyes (Rr) had children with someone who also had hybrid round.

Heredity Obj. 3d. Heredity The passing of traits from parent to offspring Traits: physical characteristics of an organism –Example: eye color, hair color,

1 Mendelian Genetics. 2 Gregor Mendel The Father of Genetics.

Heredity and Genetics Who do you look like?.

Genotypes & Phenotypes

Current position of MALDI-TOF MS in the workflow of the clinical microbiology laboratory, including the current options for analysis of bacteria directly.

Distributions of parvovirus B19 genotype 1-3 in blood donations

S. M. Arend, E. Cerdé de Palou, P. de Haas, R. Janssen, M. A. Hoeve, E

E. Lecorche, S. Haenn, F. Mougari, S. Kumanski, N. Veziris, H

Morphology of M. leprae. Morphology of M. leprae. A. M. leprae is weakly acid fast but, when stained with the Fite-Faraco method, it appears as red, rod-shaped.

Circular map of the chromosome of M. tuberculosis H37Rv.

Itraconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

SPLITTING OF COMBINED SHAPES

Amino acid alignment of TprK showing six different sequences in the T

Timeline of major events in influenza vaccine development.

Dihybrid Crosses.

Phylogenetic tree showing generic and species positions based on Bayesian analysis of the nuclear ribosomal DNA 28S region (1,200 bp) of Schistosomatidae.

Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer,

RND efflux operons in P. aeruginosa.

Neighbor-joining dendrogram based on concatenated gene fragments of adk, atpG, frdB, mdh, pgi, and recA (2,712 nucleotides), comparing the type strains.

Antiviral drug groups for the treatment of 9 infectious diseases.

Punnett Squares.

Relative abundances of Propionibacterium species in different skin areas determined by 16S rRNA gene sequence analysis of 10 individuals. Relative abundances.

Strategies for the detection of Salmonella and Shigella on routine stool cultures. Strategies for the detection of Salmonella and Shigella on routine stool.

Verigene solid-phase microarray.

Phylogenetic tree. Phylogenetic tree. Shown are circle phylogram relationships for known genotypes of HRV-A, HRV-B, and HRV-C. The tree was calculated.

Rectangle, x = 100, y = 200 Ellipse, x = 200, y = 300 #1.

Technology behind novel diagnostic methods for fungal infections.

Structure of PrfA (A) and its target DNA sequences in PrfA-regulated promoters (B). Structure of PrfA (A) and its target DNA sequences in PrfA-regulated.

Fluconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

Number of outbreaks associated with drinking water by water system type and year (n = 780), 1971 to “Other” includes outbreaks associated with bottled.

Percentages of outbreak deficiencies (n = 671) in public water systems (n = 656) by time period, 1971 to 2006, excluding outbreaks associated with Legionella.

Transcription-mediated amplification (TMA).

xTAG liquid-phase microarray.

Β-Lactam hydrolysis of cephalosporin followed by nonenzymatic release of a leaving group (LG). β-Lactam hydrolysis of cephalosporin followed by nonenzymatic.

Examples of advanced hybrid antibiotics undergoing clinical trials: cadazolid (hybrid 17) and TNP-2092, also known as CBR-2092 (hybrid 18). Examples of.

Number of outbreaks associated with drinking water by system type and month (n = 762), 1971 to 2006, excluding outbreaks associated with commercially bottled.

Summary of the distribution and genetic context of the OXA-type enzymes in Acinetobacter baumannii. Summary of the distribution and genetic context of.

Fabrizio Dutly, and Martin Altwegg Clin. Microbiol. Rev. 2001; doi:10

Staphylococcus aureus and Staphylococcus epidermidis cell surface proteins, known as microbial surface components recognizing adhesive matrix molecules.

Structure of cefiderocol (hybrid 10), previously known as S , derived by linking ceftazidime to the siderophore catechol 2-chloro-3,4-dihydroxybenzoic.

Phylogenetic tree representation of a neighbor-joining analysis of several species of piroplasms. Phylogenetic tree representation of a neighbor-joining.

Hector H. García et al. Clin. Microbiol. Rev. 2002; doi: /CMR

Workflow summary of printed microarrays.

Recovery template. Recovery template. The recovery template (internal control) has the same sequence as the PCR product except the probe region has been.

Roberta B. Carey et al. Clin. Microbiol. Rev. 2018; doi: /CMR

Schematic representation of 5′→3′ exonuclease cleavage of a 5′-labeled (small black star) probe with a 3′-phosphate (grey circle) extension blocker. Schematic.

Sequence of helix 18 of the 16S rRNA genes (corresponding to Escherichia coli positions 430 to 500) of different Mycobacterium species. Sequence of helix.

Synchronization of a basic diagnostic algorithm (linked diagnostic tests) with different levels of diagnostic services. Synchronization of a basic diagnostic.

The future TB clinic. The future TB clinic. Sputum, saliva, and fecal samples are collected from patients for genome sequencing, SNP typing, or metagenomic.

Simplified representation of the reverse genetic approach used to construct the chimera vaccine Poulvac FluFend i AI H5N3 RG to protect poultry against.

Bacterial architecture and targets for biosensing.

Number of ESBL carriers in the community in 2010, according to WHO region grouping. Number of ESBL carriers in the community in 2010, according to WHO.

The Scorpions primer is an extension of molecular beacons.

Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. Integration of the kDNA minicircle sequence into the genome.

Southern blot demonstrating allelic heterozygosity of the vspC5 gene as well as members of the family of genes related to vspC5. Southern blot demonstrating.

Phylogenetic trees for fusion (F) and attachment (G) genes of selected HMPV isolates. Phylogenetic trees for fusion (F) and attachment (G) genes of selected.

Chromosomal maps of three M

Genotyping results of Xusw47 (banding patterns; 220 bp: high Cd, 350 bp: low Cd) and designed KASPar of IWB47298 (letters at the bottom; T: low Cd, C:

Pathology of acute human Chagas' disease.

Affymetrix GeneChip oligonucleotide microarray.

Basic diagnostic algorithm to link the molecular line probe assay with solid culture- and liquid culture-based growth detection and susceptibility testing.

Significant differences in target copy numbers in multiplex PCRs may result in incorrect identification at high Cq values. Significant differences in target.

SPI-1 regulatory network.

Outcomes of infections caused by carbapenemase-producing Klebsiella pneumoniae, according to treatment regimen. Outcomes of infections caused by carbapenemase-producing.

Characterization of CWR22 xenografts and XMRV-related sequences.

Amino acid substitutions in SHV ESBL derivatives.

Presentation transcript:

Identification algorithm combining the use of commercial DNA probes (rounded rectangles and squares) and genetic sequencing (ellipses). Identification algorithm combining the use of commercial DNA probes (rounded rectangles and squares) and genetic sequencing (ellipses). For hybridization patterns known to be subject to cross-reactions (rounded rectangles and squares) with different commercial DNA probes (AccuProbe, blue-gray; INNO-LiPA Mycobacteria, gray; GenoType Mycobacterium, light blue), the appropriate sequencing targets are suggested. The hybridization patterns are indicated by the positive probes (probe names are according to the package inserts of different kits). Enrico Tortoli Clin. Microbiol. Rev. 2014; doi:10.1128/CMR.00035-14