Oocyte meiotic-stage-specific differences in spindle depolymerization in response to temperature changes monitored with polarized field microscopy and.

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Oocyte meiotic-stage-specific differences in spindle depolymerization in response to temperature changes monitored with polarized field microscopy and immunocytochemistry Claudia Gomes, M.D., Ph.D., Mariana Merlini, B.Sc., Jeremy Konheim, B.Sc., Paulo Serafini, M.D., Ph.D., Eduardo L.A. Motta, M.D., Ph.D., Edmund C. Baracat, M.D., Ph.D., Gary D. Smith, Ph.D. Fertility and Sterility Volume 97, Issue 3, Pages 714-719 (March 2012) DOI: 10.1016/j.fertnstert.2011.12.018 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 The presence and absence of tubulin polymerization in mouse oocytes detected using ICC and PFM. Using ICC, polymerized and depolymerized meiotic spindles were detected in MI oocytes (A1 and A3, respectively), TI oocytes (B1 and B3, respectively), and MII oocytes (C1 and C3, respectively). Using PFM, black-and-white images were obtained of polymerized and depolymerized meiotic spindles in MI oocytes (A2 and A4, respectively), TI oocytes (B2 and B4, respectively), and MII oocytes (C2 and C4, respectively). dPB = divided polar body; PB = polar body. All images are at a magnification of ×400, except for the inset boxes shown for the A1, B1, and C1 panels that represent images of MI, TI, and MII, respectively, under light microscope. The dotted outlines indicate the polar body membrane. Fertility and Sterility 2012 97, 714-719DOI: (10.1016/j.fertnstert.2011.12.018) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Percentages of meiotic spindles visualized in metaphase I oocytes analyzed by PFM and ICC. Oocytes were incubated at 37°C, RT, or 4°C for 0, 10, 30, and 60 minutes before being assayed. Fertility and Sterility 2012 97, 714-719DOI: (10.1016/j.fertnstert.2011.12.018) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Percentages of meiotic spindles visualized in telophase I oocytes analyzed by PFM and ICC. Oocytes were incubated at 37°C, RT, or 4°C for 0, 10, 30, and 60 minutes before being assayed. NS = not significant. Fertility and Sterility 2012 97, 714-719DOI: (10.1016/j.fertnstert.2011.12.018) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Percentages of meiotic spindles visualized in metaphase II oocytes analyzed by PFM and ICC. Oocytes were incubated at 37°C, RT, or 4°C for 0, 10, 30, and 60 minutes before being assayed. Fertility and Sterility 2012 97, 714-719DOI: (10.1016/j.fertnstert.2011.12.018) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions