A, iDC (top) or mDC (bottom) were loaded with or without 10 pfu/cell reovirus, and cultured with Mel-888 cells in the presence of blocking serum. A, iDC.

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A, iDC (top) or mDC (bottom) were loaded with or without 10 pfu/cell reovirus, and cultured with Mel-888 cells in the presence of blocking serum. A, iDC (top) or mDC (bottom) were loaded with or without 10 pfu/cell reovirus, and cultured with Mel-888 cells in the presence of blocking serum. After 48 hours, the cells were harvested, labeled with CD11c, and analyzed for the maturation markers indicated. Plots shown are representative of 3 independent experiments. B, PFA-fixed or live DC were loaded as in (A) and cultured with Cell Tracker Green-labeled Mel-888 cells, then harvested and labeled with CD11c. Double-positive cells were identified by FACS. Plots shown are representative of 4 independent experiments. C, reovirus-loaded cells [iDC(reo), mDC(reo)] or nonloaded cells (iDC, mDC), were cultured for 24 hours with Mel-888 cells and used to prime isolated autologous T cells. Cells were harvested and a CD107 degranulation assay against Mel-888 or SKOV-3 (irrelevant) targets was conducted. Plots shown are representative of 3 independent experiments; numbers indicate percentage of CD8 cells degranulating. Elizabeth J. Ilett et al. Clin Cancer Res 2011;17:2767-2776 ©2011 by American Association for Cancer Research