Mechanism of polymerization inhibition by increasing oxygen affinity.

Slides:

Advertisements

Similar presentations

CO2 Transport Carbon dioxide transport: Carbon dioxide is produced by cells throughout the body. It diffuses out of the cells and into the systemic capillaries,

Advertisements

Oxygen Binding Proteins

Protein Function Structure will determine the function of the protein.

The heme group..

Dr. Nasim.  Hemeproteins are a group of specialized proteins that contain heme as a tightly bound prosthetic group  hemoglobin and myoglobin, the two.

Oxygen Binding Proteins

Transport of O2 and CO2 by hemoglobin

Proteins serve a variety of functions. Transport –Myoglobin transports O 2 throughout muscles. –Hemoglobin transports O 2 in blood. Structural –Actin forms.

Lecture 14: Regulation of Proteins 1: Allosteric Control of ATCase

Hemoglobin Tetramer [Hb(O 2 )] [Hb]P O 2 K 2 = [Hb(O 2 )] [Hb]P O 2 K 3 = [Hb(O 2 )] [Hb]P O 2 K 4 = [Hb(O 2 )] [Hb]P O 2 K 1 = = 4.88 = 15.4 = 6.49 =

Diffusion is the movement of molecules from a high concentration to a low concentration. Many molecules diffuse across cell membranes.

QUATERNARY STRUCTURE OF PROTEINS Arrangement of chains in a multichain protein Noncovalent association for globular proteins Covalent association likely.

Relationship between the structure and function of proteins.

Blood Disorders. Methemoglobinemia a disorder characterized by the presence of a higher than normal level of methemoglobin (metHb) in the bloodmethemoglobinblood.

ROMA 3 March 2006, Pier Luigi Luisi LECTURE nr. 10 WHY IS THIS...AND NOT THAT? WHY ARE ENZYMES SO LARGE?

Oxygen Transport and Asphyxia AnS 536 Spring 2016.

Myoglobin (Mb) and Hemoglobin (Hb) have related, but different, roles in the body Hemoglobin: Found in red blood cells Promotes diffusion of O 2 throughout.

Biochemistry Kay Iwelumo. Objectives Proteins- Composition/Structure Protein Purification Enzymes- Kinetics and Inhibition Hemoglobin/Myoglobin Membrane-

Sickle Cell Anemia Most common genetic disease in US

Volume 112, Issue 12, Pages (June 2017)

Volume 20, Issue 8, Pages (August 2012)

Thanks for the complement (inhibitor)‏

A Clinical Evaluation of the International Lymphoma Study Group Classification of Non-Hodgkin's Lymphoma Blood Volume 89(11): June 1, 1997 ©1997.

Volume 13, Issue 4, Pages (April 2008)

Volume 104, Issue 11, Pages (June 2013)

Zinc fingers poke zebrafish, cause thrombosis!

Structure of unliganded HIV-1 reverse transcriptase at 2

Shiqing Li, Paul Kussie, Kathryn M. Ferguson Structure

A new red cell shape helps the clot

Module 2 Exchange and transport

Analysis of glutathione S-transferase allergen cross-reactivity in a North American population: Relevance for molecular diagnosis Geoffrey A. Mueller,

Rong Shi, Laura McDonald, Miroslaw Cygler, Irena Ekiel Structure

Hemoglobin and Myoglobin

Volume 21, Issue 8, Pages (August 2013)

Chapter 01 Introduction.

Volume 21, Issue 6, Pages (June 2013)

G. Fiorin, A. Pastore, P. Carloni, M. Parrinello Biophysical Journal

Dániel Szöllősi, Gergely Szakács, Peter Chiba, Thomas Stockner

Mechanism of Sirtuin Inhibition by Nicotinamide: Altering the NAD+ Cosubstrate Specificity of a Sir2 Enzyme José L. Avalos, Katherine M. Bever, Cynthia.

Volume 9, Issue 5, Pages (May 2002)

Regulation of protein phosphatase-1

AMPA Receptor Activation

Cell Size and Diffusion

Two structurally different RNA molecules are bound by the spliceosomal protein U1A using the same recognition strategy Luca Jovine, Chris Oubridge, Johanna.

Volume 85, Issue 4, Pages (October 2003)

Volume 7, Issue 4, Pages (April 2005)

Volume 91, Issue 5, Pages (November 1997)

A Drug-Drug Interaction Crystallizes a New Entry Point into the UPR

AMPA Receptor Activation

Volume 109, Issue 6, Pages (September 2015)

Masaru Goto, Rie Omi, Noriko Nakagawa, Ikuko Miyahara, Ken Hirotsu

RLR domains and signaling.

Volume 24, Issue 5, Pages (May 2016)

Volume 21, Issue 4, Pages (April 2013)

Volume 20, Issue 1, Pages (January 2012)

Ion-Induced Defect Permeation of Lipid Membranes

Volume 91, Issue 5, Pages (November 1997)

Chap 10. Conformational Change, Allosteric Regulation, Motors, and Work Conformation changed for controlling the activity of regulatory proteins for interconverting.

Changes throughout time in the lineage content of clones regenerated in secondary mice. Changes throughout time in the lineage content of clones regenerated.

Allosteric drugs: thinking outside the active-site box

Volume 21, Issue 4, Pages (April 2013)

Volume 22, Issue 6, Pages (June 2014)

Volume 11, Issue 2, Pages (February 2003)

Cardiolipin Interactions with Proteins

Interaction of platelet-decorated VWF strings with leukocytes and infected red blood cells in vitro. Interaction of platelet-decorated VWF strings with.

Animal navigation: Birds as geometers?

The von Willebrand factor D′D3 assembly and structural principles for factor VIII binding and concatemer biogenesis by Xianchi Dong, Nina C. Leksa, Ekta.

Annual hospital charges and costs for CEA and CAS

Volume 21, Issue 12, Pages (December 2013)

Presentation transcript:

Mechanism of polymerization inhibition by increasing oxygen affinity. Mechanism of polymerization inhibition by increasing oxygen affinity. (A) Hemoglobin exists in a rapidly reversible equilibrium between low- and high-affinity quaternary conformations, called T and R, respectively.65,66 They differ primarily by an ∼15° relative rotation of αβ dimers. Location of β6 valine is shown as a yellow dot on the surface of the molecule. Preferential binding of a small molecule such as a drug (red circle) to R shifts the quaternary equilibrium toward R. (B) Cartoon of polymerization equilibrium. Only the T quaternary structure (empty circles) enters the fiber. R quaternary conformations (filled circles) are completely excluded.69 (C) Oxygen binding curves. Preferential binding of a drug to the R quaternary structure causes a left shift (increased oxygen affinity). William A. Eaton, and H. Franklin Bunn Blood 2017;129:2719-2726 ©2017 by American Society of Hematology