Altered circulating levels of matrix metalloproteinases 2 and 9 and their inhibitors and effect of progesterone supplementation in women with endometriosis.

Slides:

Advertisements

Similar presentations

Age-specific serum anti-Müllerian hormone values for 17,120 women presenting to fertility centers within the United States David B. Seifer, M.D., Valerie.

Advertisements

What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.

Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation Malathy Appasamy,

High concentrations of lactoferrin in the follicular fluid correlate with embryo quality during in vitro fertilization cycles Atsushi Yanaihara, M.D.,

Association of functional genetic variants of CTLA4 with reduced serum CTLA4 protein levels and increased risk of idiopathic recurrent miscarriages Maneesh.

Increased expression of c-fos protein associated with increased matrix metalloproteinase-9 protein expression in the endometrium of endometriotic patients

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm Luis.

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

The relative effects of hormones and relationship factors on sexual function of women through the natural menopausal transition Lorraine Dennerstein,

Analysis of follicular fluid and serum markers of oxidative stress in women with infertility related to endometriosis Laura Prieto, M.D., Juan F. Quesada,

High soluble CD44 concentration in peritoneal fluid in endometriosis

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Endometrial expression and in vitro modulation of the iron transporter divalent metal transporter-1: implications for endometriosis Carlos Patricio Alvarado-Díaz,

Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor β-3 Doina S. Joseph, M.S., Minnie Malik, Ph.D., Sahadat.

Changes in serum anti-Müllerian hormone levels may predict damage to residual normal ovarian tissue after laparoscopic surgery for women with ovarian.

How old is too old? Challenges faced by clinicians concerning age cutoffs for patients undergoing in vitro fertilization Robert L. Klitzman, M.D. Fertility.

Trends in clinical reproductive medicine research: 10 years of growth

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

The measurements of RAGE, VEGF, and AGEs in the plasma and follicular fluid of reproductive women: the influence of aging Eriko Y. Fujii, M.D., Ph.D.,

Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in.

Oocyte cryopreservation

Prolonged gonadotropin-releasing hormone agonist therapy reduced expression of nitric oxide synthase in the endometrium of women with endometriosis and.

Two interesting cases of ovarian pregnancy after in vitro fertilization–embryo transfer and its successful laparoscopic management Selvaraj Priya, M.D.,

Follicular fluid and serum levels of Inhibin A and pregnancy-associated plasma protein A in patients undergoing IVF Jiri Moos, Ph.D., Vanda Filova, Ph.D.,

Angela R. Baerwald, Ph. D. , Olufemi A. Olatunbosun, M. D. , Roger A

Reply of the Authors Fertility and Sterility

Effects of acupuncture on pregnancy rates in women undergoing in vitro fertilization: a systematic review and meta-analysis Cui Hong Zheng, M.D., Ph.D.,

Robert F. Casper, M.D. Fertility and Sterility

Rahi Victory, M. D. , F. R. C. S. C. , Ghassan M. Saed, Ph. D

A comparison of three downregulation approaches for poor responders undergoing in vitro fertilization Laura Detti, M.D., Daniel B. Williams, M.D., Jared.

Growth differentiation factor 9 concentration in the follicular fluid of infertile women with endometriosis Hendy Hendarto, Ph.D., Prajitno Prabowo,

Increased progesterone receptor expression in uterine leiomyoma: correlation with age, number of leiomyomas, and clinical symptoms Anastasia Tsigkou,

Requirement of heart and neural crest derivatives–expressed transcript 2 during decidualization of human endometrial stromal cells in vitro Hisayuu Shindoh,

Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial cells exposed to 2,3,7,8-

Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo.

Aimee S. Browne, M. D. , M. Sc. , Jie Yu, M. D. , M. Sc

Peritoneal cytokines and adhesion formation in endometriosis: an inverse association with vascular endothelial growth factor concentration Ewa Barcz,

Introduction Fertility and Sterility

Immature oocyte retrieval and in vitro oocyte maturation at different phases of the menstrual cycle in women with cancer who require urgent gonadotoxic.

Granulosa–lutein cell growth differentiation factor-9 (GDF-9) messenger RNA and protein expression in in vitro fertilization (IVF) cycles: relation to.

Anti-Müllerian hormone and inhibin B as predictors of pregnancy after treatment by in vitro fertilization/intracytoplasmic sperm injection Dorothea M.

Diminished intrafollicular estradiol levels in in vitro fertilization cycles from women with reduced ovarian response to recombinant human follicle-stimulating.

Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence

Follicular-fluid neurotrophin levels in women undergoing assisted reproductive technology for different etiologies of infertility Erkan Buyuk, M.D.,

Tolga B. Mesen, M. D. , Lamya Kacemi-Bourhim, M. D. , Paul B

Kirstine Kirkegaard, M. D. , Johnny Juhl Hindkjaer, M. Sc

Peritoneal fluid concentrations of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and transforming growth factor-beta in women with.

Prospective cohort study of three- versus two-dimensional ultrasound for prediction of oocyte maturity Doron Shmorgun, M.D., Edward Hughes, M.D., Patrick.

Endocrine gland–derived vascular endothelial growth factor concentrations in follicular fluid and serum may predict ovarian hyperstimulation syndrome.

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

S. Samuel Kim, M.D. Fertility and Sterility

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Low serum and peritoneal fluid concentration of interferon-γ–induced protein-10 (CXCL10) in women with endometriosis Letizia Galleri, Ph.D., Stefano.

Circulating microRNAs as potential biomarkers for endometriosis

Fertility and Sterility: an evaluation

Impact of subclinical hypothyroidism in women with recurrent early pregnancy loss Lia A. Bernardi, M.D., Ronald N. Cohen, M.D., Mary D. Stephenson, M.D.,

Effects of gonadotropin-releasing hormone agonist/recombinant follicle-stimulating hormone versus gonadotropin-releasing hormone antagonist/recombinant.

Eric S. Surrey, M. D. , Debra A. Minjarez, M. D. , John M. Stevens, B

Genetic evaluation procedures at sperm banks in the United States

Production of macrophage inflammatory protein-3α in human follicular fluid and cultured granulosa cells Yasushi Kawano, M.D., Junichiro Fukuda, M.D.,

Follicular fluid of single follicles from women with PCOS contains higher levels of müllerian inhibiting substance L.T. Ku, S.T. Nakajima, M.E. McClure,

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Serum levels of soluble vascular cell adhesion molecule-1 are decreased in women receiving oral contraceptives compared with normally menstruating women:

Soluble HLA-G in the peritoneal fluid of women with endometriosis

Transforming growth factor-β1 and its receptor soluble endoglin are altered in polycystic ovary syndrome during controlled ovarian stimulation Reshef.

What’s the delay? A qualitative study of women’s experiences of reaching a diagnosis of endometriosis Karen Ballard, Ph.D., Karen Lowton, Ph.D., Jeremy.

Ovarian follicular volume and follicular surface area are better indicators of follicular growth and maturation, respectively, than is follicular diameter

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

In vitro viability and secretory capacity of human luteinized granulosa cells after gonadotropin-releasing hormone agonist trigger of oocyte maturation

Maternal serum screening markers and nuchal translucency measurements in in vitro fertilization pregnancies: a systematic review Andrea Lanes, M.Sc.,

Presentation transcript:

Altered circulating levels of matrix metalloproteinases 2 and 9 and their inhibitors and effect of progesterone supplementation in women with endometriosis undergoing in vitro fertilization Abhay Kumar Singh, M.Sc., Ratna Chattopadhyay, M.B.B.S., Baidyanath Chakravarty, M.D., Koel Chaudhury, Ph.D. Fertility and Sterility Volume 100, Issue 1, Pages 127-134.e1 (July 2013) DOI: 10.1016/j.fertnstert.2013.03.006 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 The bar graphs show levels of matrix metalloproteinase (MMP) 2, MMP-9, tissue inhibitor of MMP (TIMP) 1, TIMP-2 (ng/mL), and transforming growth factor (TGF) β1 (pg/mL) in (A) follicular fluid and (B) serum of group A compared with group B. A representative Western blot analysis of Smad-2 and Smad-3 in (C) follicular fluid and (D) serum is shown for both the groups. β-Actin expression was used to normalize the amount of loaded protein in each well. The bar graphs represent two independent experiments obtained by densitometric scanning of the blots. Values are expressed as mean ± SD. *P≤.05. Fertility and Sterility 2013 100, 127-134.e1DOI: (10.1016/j.fertnstert.2013.03.006) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Follicular fluid concentrations of (A) MMP-2, (B) MMP-9, (C) TIMP-1, and (D) TIMP-2 of follicles containing different grades of oocytes (mature and immature) and embryo quality (grade I+II and grade III) in women of group A. The box and whisker plots are constructed from five values: the smallest value, first quartile, median, third quartile, and maximum values. Abbreviations as in Figure 1. Fertility and Sterility 2013 100, 127-134.e1DOI: (10.1016/j.fertnstert.2013.03.006) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Comparison of serum MMP-2, MMP-9, TIMP-1, and TIMP-2 levels in pregnant women of group A before (BPS) and after (APS) P supplementation (A; *P<.001). Mean (±SD) values of MMP-9/TIMP-1 ratio are shown in (B) and MMP-2/TIMP-2 ratio in (C) before and after progesterone supplementation to pregnant women of both the groups (*P<.05; Mann-Whitney U test). Other abbreviations as in Figure 1. Fertility and Sterility 2013 100, 127-134.e1DOI: (10.1016/j.fertnstert.2013.03.006) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions