Fig. 4 Prematurely short S-phase and replication-associated DNA damage in p57KIP2-deficient fetal liver. Prematurely short S-phase and replication-associated.

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Fig. 4 Prematurely short S-phase and replication-associated DNA damage in p57KIP2-deficient fetal liver. Prematurely short S-phase and replication-associated DNA damage in p57KIP2-deficient fetal liver. (A) Premature increase in intra–S-phase DNA synthesis rate, measured as BrdU MFI, in p57KIP2-deficient embryos. Representative cell cycle analysis of p57KIP2+/−m and wild-type littermate embryos. Embryos were pulsed in vivo with BrdU for 30 min before fetal livers were explanted. (B) Premature increase in intra–S-phase DNA synthesis rate in p57KIP2-deficient embryos. Data summary, analyzed as in (A); n = 29 (+/+), 12 (−/−), and 18 (+/−m). For each embryo, S-phase BrdU MFI in S0 is expressed as a percentage of S-phase BrdU MFI in S1 of the same fetal liver. (C) Increased γH2AX in S0 cells of p57KIP2-deficient embryos. Representative examples of freshly explanted fetal livers of p57KIP2-deficient embryos and wild-type littermates, labeled with an antibody against γH2AX. DNA content was measured using 7-amino-actinomycin D (7AAD). See also fig. S3. (D) Increased γH2AX in S0 cells of p57KIP2-deficient embryos. Summary of data obtained as in (C) for a total of n = 79 embryos. γH2AX measured in arbitrary fluorescence units. (E) Distribution of γH2AX labeling, DNA content, and BrdU incorporation in G1, S, or G2-M phases of the cell cycle and in γH2AX-positive cells, all in the S0 subset of a single p57KIP2−/− fetal liver. Embryos were pulsed in vivo with BrdU for 30 min, and fetal livers were harvested, fixed, and labeled for BrdU incorporation, DNA, and γH2AX. S0 cells were subdivided digitally into cell cycle phase gates based on their DNA content and BrdU incorporation. Cells positive for γH2AX were gated based only on γH2AX signal regardless of cell cycle phase. (F) p57KIP2-deficient γH2AX-positive S0 cells are slowed or arrested in S phase. Data summary for 17 p57KIP2−/+m fetal livers, analyzed as in (E). Each data point is BrdU MFI (bottom) or median DNA content (top) for all cells in a specific category (G1, S, G2-M, or γH2AX-positive) in a single fetal liver. BrdU MFI and median DNA content were normalized to their respective values in G1 cells of the S0 subset in each fetal liver. Yung Hwang et al. Sci Adv 2017;3:e1700298 Copyright © 2017, The Authors