Processive movement of single kinesins on crowded microtubules visualized using quantum dots Effect of the mutant E164A on the motility of single quantum.

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Processive movement of single kinesins on crowded microtubules visualized using quantum dots Effect of the mutant E164A on the motility of single quantum dot‐labelled wild‐type kinesins. Motility parameters of the runs of 1 nM quantum dots associated with 1 nM biotinylated wild‐type kinesins in the presence of 0–1.6 μM mutant E164A and 2 mM MgATP. (A) Binding frequency of single quantum dot‐labelled wild‐type kinesins to microtubules (black squares) and the calculated error (black bars). (B) Average velocity of single quantum dot‐labelled wild‐type kinesins, as calculated from a Gaussian fit of the velocity distribution (black squares) plotted together with the standard deviation of the fitted distribution (black bars), and the mean velocities (red circles) plotted together with their standard deviation (red bars). (C) Average run length as obtained from a fit to the probability distribution of the individual run lengths (black squares), and the mean run length (red circles) with the standard deviation (red bars). (D) Average dwell time as obtained from a fit to the probability distribution of the individual dwell times (black squares) and the error of the fit parameter (black bars). The red lines are fits to the kinetic parameters at different mutant E164A concentrations based on the kinetic model illustrated in Figure 5 and as described in Materials and methods. Note the different scale of the abscissa as compared to Figure 3. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 25, Issue: 2, Pages: 267-277, First published: 12 January 2006, DOI: (10.1038/sj.emboj.7600937)