Expression of CRC stem cell markers and L1 in CRC cells.

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Expression of CRC stem cell markers and L1 in CRC cells. Expression of CRC stem cell markers and L1 in CRC cells. A, HCT116 cells were sorted by FACS using antibody to CD133 (left), and analyzed by Western blotting for the expression of L1, CD133, CD44, and EpCAM (right). Tubulin was used as loading control. B, SW620 cells were separated by FACS into CD133+ and CD133− cell populations as in A (left), and analyzed by Western blotting for the expression of E-cadherin and EpCAM (right). The levels of CD133 and L1 RNA (C, left) and protein (C, right) were determined in single cell clones of HCT116 and SW620 CRC cells in which the levels of L1 were modulated by overexpression (HCT116), or were suppressed by L1 shRNA (SW620). GAPDH served as control for RNA levels and tubulin as protein loading control. Nancy Gavert et al. Mol Cancer Res 2011;9:14-24 ©2011 by American Association for Cancer Research