Evolved changes in the LPS pattern and the causative mutation.

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Evolved changes in the LPS pattern and the causative mutation. Evolved changes in the LPS pattern and the causative mutation. (Left) Electrophoretic profiles of LPSs extracted from B. multivorans isolates representative of all clades and wbi alleles, in which the wgiG mutation resulting in P169L and a frameshift mutation in wbiI are associated with the loss of the O antigen. (Right) BM2 and BM3 were complemented with the empty vector (pBBR1MCS) or a vector carrying a functional wgiG copy from isolate BM1, which restores the O antigen. Lanes M, protein markers. WT, wild-type allele; *, wbiI frameshift mutation. Inês N. Silva et al. mSystems 2016; doi:10.1128/mSystems.00029-16