Mammalian cell cytotoxicity shown by macrophage response to DM262.

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Figure 1 C. LDH release D. Cell viability E. Cell proliferation

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Mammalian cell cytotoxicity shown by macrophage response to DM262. Mammalian cell cytotoxicity shown by macrophage response to DM262. Viability was measured in BMDM and J774 cells using an MTT assay on DM262 which is shown to have an LD50 of 12.5 μM. Cell lysis was measured by LDH release into the supernatant of BMDM, showing similar numbers with an LD50 of 12.5 μM. Data points were determined in triplicate, and this experiment was repeated twice. Shown are mean and standard deviation for each value. Eric H. Jung et al. mSphere 2018; doi:10.1128/mSphere.00537-17