Fig. 4. Flow cytometric analysis of the blocking potency of purified camel anti-SLP-MAPS isotypes to the binding of huIgE to its high affinity receptor.

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Fig. 4. Flow cytometric analysis of the blocking potency of purified camel anti-SLP-MAPS isotypes to the binding of huIgE to its high affinity receptor (FcεRI) on stripped human basophils. A1-A4 & B1-B4. Fluorescence of anti-IgE-FITC to atopic serum-sensitized human basophils. In A1, atopic serum and camel isotypes are absent. B1 is a complete system in the absence of camel isotypes. B2-B4 Basophils sensitization in the presence of postimmunized camel IgG1 (B2), IgG2 (B3), and IgG3 (B4). A2-A4 Basophils sensitization in the presence of preimmunized camel IgG1 (A2), IgG2 (A3), and IgG3 (A4). Numbers in quadrants represent the percentages of IgE-positive basophils. Blot in the upper left corner of the histogram represent relevant dot blot. Fig. 4. Flow cytometric analysis of the blocking potency of purified camel anti-SLP-MAPS isotypes to the binding of huIgE to its high affinity receptor (FcεRI) on stripped human basophils. A1-A4 & B1-B4. Fluorescence of anti-IgE-FITC to atopic serum-sensitized human basophils. In A1, atopic serum and camel isotypes are absent. B1 is a complete system in the absence of camel . . . Allergy Asthma Immunol Res. 2015 Nov;7(6):583-589. http://dx.doi.org/10.4168/aair.2015.7.6.583