A tph2 promoter fragment labels DR serotonergic neurons.

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A tph2 promoter fragment labels DR serotonergic neurons. A tph2 promoter fragment labels DR serotonergic neurons. A, Genomic organization of the tph2 gene upstream region from which the 3.4 kb promoter was taken (brown bar) and schematic of the construct used to create the stable transgenic Tg(tph2:Gal4ff)y228 line. B, C, Whole-body lateral (B) and oblique (C) head views of 6 dpf Tg(tph2:Gal4ff)y228; Tg(UAS:Kaede)s1999t double-transgenic larva showing Kaede expression in the ventral midline of the brainstem (arrowheads) and spinal cord (asterisks). Dotted line in C outlines the brain. D, Immunohistochemistry against serotonin (red) confirms that Kaede-expressing cells in the double-transgenic line are serotonergic. Anterior is to the left. The posterior extension of serotonergic neurons that are not labeled in Tg(tph2:Gal4ff)y228 is the inferior raphe. A, Anterior; D, dorsal; L, left; P, posterior; R, right; V, ventral. Scale bar, 50 μm. Tohei Yokogawa et al. J. Neurosci. 2012;32:15205-15215 ©2012 by Society for Neuroscience