Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic.

Slides:

Advertisements

Similar presentations

Targeting the PARP DNA repair pathway enhanced cytotoxicity induced by chemotherapy. Targeting the PARP DNA repair pathway enhanced cytotoxicity induced.

Advertisements

IDH1 mutant glioma cells are sensitive to TMZ

Cytotoxic effects of cAb-carcinoembryonic antigen 5 (CEA5)::β-lactamase (βL) + 7-(4-carboxybutanamido) cephalosporin mustard (CCM; ♦) combinations on LS174T.

Figure 2 Logistical requirements for autologous

A, B, apoptosis analysis using Annexin V-FITC/PI was done on 5th, 7th, and 9th day after transfection either with si control or si AEBP1in U87MG and U138MG.

Antigen specificity of direct tumor recognition by HTLV-1 Tax–reactive HTLs. The peptide Tax reactive HTLs 5G, 8E, and 9B (A-C) and peptide Tax reactive.

Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.

Cytotoxic activity of HER2-lytic hybrid peptide.

A, ATP production by PC3 prostate cancer cells after treatment with oligonucleotide/Lipofectin complexes. a, ATP production by PC3 prostate cancer cells.

Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific.

Dietary plant extracts inhibit NF-κB activation in vitro.

G3139 substitutes for heparin, which is required for the biological activity of FGF2. G3139 substitutes for heparin, which is required for the biological.

The presence of soluble IL12p70 during moDCpoly stimulation of KIRnegNKG2Aneg cells results in a dominant population of multifunctional NKG2Apos NK cells.

Frequent alterations identified and potential actionability.

Colony-forming capacity of CD34+ cells with and without CLL-1 expression. Colony-forming capacity of CD34+ cells with and without CLL-1 expression. CD34+

Cytotoxicity of p5399–107-induced CTL cells.

In vivo growth-inhibitory effect of perifosine on bevacizumab-resistant CCC. A, the effect of bevacizumab (BEV) on CCC growth in vivo. In vivo growth-inhibitory.

Anti-Flk-1 treatment inhibits growth of s. c. 4T1 and B16 tumors. s. c

FOXO1 inhibits Runx2-induced invasion of prostate cancer cells.

SiRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. siRNA silencing of CDH3,

Direct recognition of MHC II/TARP14-27 on tumor cells by HTL

A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.

Specific cytotoxicity of DTEGF13.

HLA-DR53–restricted HTL responses to peptide TARP1-14.

Induction of cytotoxic activity in humanized SCC3 tumor-bearing mice treated with anti-PD-1 antibody. Induction of cytotoxic activity in humanized SCC3.

Immune responses to 12MP and 6MHP.

Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline. Biological effects of.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Tipifarnib and bortezomib are synergistic in cytotoxicity assays

Synergistic cytotoxicity of 17AAG and TNF treatment in human lung cancer cell lines. Synergistic cytotoxicity of 17AAG and TNF treatment in human lung.

Immunologic and pharmacokinetic studies.

A protracted ketogenic diet increases radiation response in H292 lung cancer xenografts given conventional fractionated radiation. A protracted ketogenic.

Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.

NKT cell recognition of CD1d1 molecules is inhibited by treatment with ascites. NKT cell recognition of CD1d1 molecules is inhibited by treatment with.

Increased levels of acetylated histone H3 are observed in normal PBMCs and circulating tumor cells obtained from patients post-depsipeptide treatment.

Reduced klotho expression in pancreatic cancer.

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

Effect of siltuximab on paclitaxel sensitivity in ovarian cancer drug resistant cells. Effect of siltuximab on paclitaxel sensitivity in ovarian cancer.

Patient Tregs express normal levels of suppression.

Mean cell number in small intestinal crypt (a), small intestinal villus (b), and midcolonic crypt (c) of BALB/c (♦) and DBA/2 (▪) mice after two injections.

A and B, intratumoral DC-AdCCL21 leads to reduction in growth rates of bilateral tumors. A and B, intratumoral DC-AdCCL21 leads to reduction in growth.

PK profile of OTL78. PK profile of OTL78. A, Representative time-dependent whole-body fluorescence images over white light images of a mouse bearing 22Rv1.

Kaplan–Meier curves for RFS and OS by various marker groups.

Only IFNγ shows consistently increased mRNA expression in mycosis fungoides (MF) lesions. mRNA expression levels of various cytokines in the skin of healthy.

Mean weight change from original weight of (a) BALB/c (b) DBA/2 mice after one (♦) or two (▴) injections of 10 mg/kg and one (▪) or two (×) injections.

Characterizing an A*1101-restricted SSC-specific CD8+ cytotoxic T-cell clone. Characterizing an A*1101-restricted SSC-specific CD8+ cytotoxic T-cell clone.

The influence of diverse genotypes of rs on the expression of TRAF3 (A), IFNβ (B), in PBMCs of healthy individuals. The influence of diverse genotypes.

A, capsid modifications increase the infectivity of gastric cancer cell lines. A, capsid modifications increase the infectivity of gastric cancer cell.

Association of overall survival and PAP-specific (A, B) and PA2024-specific (C, D) CTL activity at week 26 using categorical (tertile) analysis and continuous.

Mean percentage change from baseline in total IGF-I and the R1507 serum concentration values following a single i.v. administration of R mg/kg (n.

ROC analysis of MIC-1 and CA19-9.

VEGF165 stimulates migration of HMVECs

The best response for target lesions per patient for patients with target lesions assessed at baseline and at least 1 follow-up. The best response for.

SPARC is required for spontaneous metastasis

A, cell number was assessed 96 hours after exposure to indicated treatment in indicated cell models. A, cell number was assessed 96 hours after exposure.

The influence of diverse genotypes of rs on the expression of MAVS (A), IFNβ (B), in PBMCs of healthy individuals. The influence of diverse genotypes.

Effect of bevacizumab on the proliferation of A2780 cells.

Proliferation of TA3-Ha and TA3-St cells in vitro and in vivo.

CpG oligodeoxynucleotide (CpG ODN)–induced activation of different types of primary malignant B cells. CpG oligodeoxynucleotide (CpG ODN)–induced activation.

Reduced expression of the tumor suppressor PTEN occurs in tumors with both squamous cell and adenocarcinoma histology. Reduced expression of the tumor.

Effect of MIF siRNA on the production of MMP-13 induced by LPA

Regression analysis showing the positive correlation between number of months since the prior chemotherapy regimen (X axis) and the difference between.

Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death. Effect of dexamethasone.

Expression of chemokine receptors in A-498 cell line.

Relative frequencies of FGFR aberrations in non–small cell lung carcinoma. Relative frequencies of FGFR aberrations in non–small cell lung carcinoma. A,

CPGL is a growth suppressor in pancreatic cancer cell lines.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

Frequency of CD4+CD25+FoxP3+ and CD4+CD25+CD127low T cells in unactivated PBMCs from healthy donors. Frequency of CD4+CD25+FoxP3+ and CD4+CD25+CD127low.

Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced proliferation in HepG2 cells. Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced.

E2F4 program is predictive of the efficacy of intravesical BCG immunotherapy in NMIBC. A, the survival curves of intravesical therapy-treated and untreated.

Presentation transcript:

Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic cells (DC) as APC. Points, triplicate determinations; bars, SD. Points without bars had SD < 10% th... Peptide dose-response curves were done to estimate the avidity of these HTL for peptide TARP1-14 (A) and TARP14-27 (B-E) using autologous PBMCs and dendritic cells (DC) as APC. Points, triplicate determinations; bars, SD. Points without bars had SD < 10% the value of the mean. Representative of at least two to three experiments that were done with the same samples. Hiroya Kobayashi et al. Clin Cancer Res 2005;11:3869-3878 ©2005 by American Association for Cancer Research