Effect of therapy with S247 on the development of liver metastases after 14 and 21 days of tumor growth. Effect of therapy with S247 on the development of liver metastases after 14 and 21 days of tumor growth. CT26 colon cancer cells were injected into the spleens of BALB/c mice. On day 7, osmotic pumps containing 70 mg/kg/day S247 or saline were implanted for continuous therapy. Additionally, a bolus of 10.75 mg/kg S247 or saline was given i.p. Mice were sacrificed on day 7 (before implantation of the osmotic pumps), on day 14, or on day 21, and liver metastases were counted (horizontal lines indicate the median number of liver metastases). S247 led to significantly fewer liver metastases at 14 and 21 days (∗P < 0.05). Niels Reinmuth et al. Cancer Res 2003;63:2079-2087 ©2003 by American Association for Cancer Research