Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. Histone modification status at the Tβ4 promoter and at the.

Slides:

Advertisements

Similar presentations

HMGA2 is a SUMOylation target.

Advertisements

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Volume 28, Issue 3, Pages (November 2007)

Identification of the host interaction partners of HEV proteins in the human liver and fetal brain cDNA libraries. Identification of the host interaction.

HS induces sustained H3K4me3 and H3K4me2 methylation at HSP22

Jun-Ho Ha, Hyo-Jun Lee, Jae-Hoon Jung, Chung-Mo Park

Reversing the TERT promoter mutation to WT reverses the active chromatin marks and alters long-range chromatin interactions. Reversing the TERT promoter.

Chromatin regulation upon recruitment of HOTAIR

NM-3 induces p21 levels and down-regulation of Cdk2 activity.

Validation of ChIP-seq reads.

Genetic and pharmacologic inactivation of KSR1 radiosensitizes A431 cells in vitro to the lethal effect of ionizing radiation. Genetic and pharmacologic.

Volume 132, Issue 6, Pages (March 2008)

An siRNA screen identifies BER pathway members as sensitizers to temozolomide (TMZ) in pediatric GBM. A and B, SJG2 and KNS42 cells were transfected with.

HIS-24 and HPL associate with promoters of antimicrobial genes.

Epigenetic Control of the S100A6 (Calcyclin) Gene Expression

Features of the LIMT gene and RNA abundance in cell lines of different tissues of origin Features of the LIMT gene and RNA abundance in cell lines of different.

Antibody specificity ascertained by 2D-PAGE Western immunoblotting (IEF) of total cellular protein extracts from the RT4 human bladder cancer cell line.

The CHCH domain is necessary for mitochondrial import of CHCHD10

The up-regulation of Galnt3 by Pi requires activation of the ERK pathway and induction of Egr1 and Etv5. The up-regulation of Galnt3 by Pi requires activation.

Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced in differentiating 22q11.2DS hiPSCs. Acetylation of histone 3 in MEF2C and GATA4 loci is enhanced.

Genetic mapping and epigenetic landscape of RUNX3 locus overlapping rs

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Fig. 4 Gene disruption via chip.

Fig. 4 p100/TSN enables E2F1 to interact with alternatively spliced transcripts. p100/TSN enables E2F1 to interact with alternatively spliced transcripts.

Fig. 5 E2F1 also interacts with alternatively spliced transcripts from the MECOM gene. E2F1 also interacts with alternatively spliced transcripts from.

A and B, mRNA expression for ZNF423 (A) and BRCA1 (B) for lymphoblastoid cell lines with WT/WT (8 cell lines), WT/V (7 cell lines), and V/V (8 cell lines)

Characteristics of a novel intronic enhancer human islet.

The MEG3 promoter and enhancer interact with other putative enhancers within the DLK1-MEG3–imprinted region. The MEG3 promoter and enhancer interact with.

GA blocks HIF activity and reduces HIF target expression.

The contributions of anti-Ad antibody and T-cell responses to viral clearance in tumor and antitumor efficacy of Ad5 therapy. The contributions of anti-Ad.

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

VP16-E2 is a more potent transcriptional activator than Gal4-VP16.

Gramicidin A (GA) decreases HIF protein expression in RCC cells.

Variation in dUTPase expression in cell lines.

PTENP1 expression is downregulated in ccRCC.

Epigenetic regulation of p16INK4a in human gastric cancer.

CDCP1 colocalizes and interacts with MT1-MMP.

Mapping the Pirh2 and p73 interaction sites.

Interaction of MAPJD with E-box sequence of the RIOK1 gene.

In vitro TDP1 catalytic activity correlates positively with TDP1 protein levels in colorectal cancer cell lines. In vitro TDP1 catalytic activity correlates.

SATB2-AS1 repressed Snail transcription depending on SATB2-mediated recruitment of HDAC1. SATB2-AS1 repressed Snail transcription depending on SATB2-mediated.

NF-kB binds to the NF-κB binding site immediately adjacent to the AT-rich regulatory region. NF-kB binds to the NF-κB binding site immediately adjacent.

MTB1 Antagonizes MYC2 for DNA Binding.

PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.

Enhancement of MYC-related HAT complex recruitment to the target genes and histone H4 acetylation by MAPJD. A, interaction of MAPJD with HAT complex containing.

EGFR and cetuximab sensitivity of SCCUAT

Effect of G9a on the expression of GSH synthesis enzymes.

TDP1 and TOP1 protein levels correlate positively with TDP1 and TOP1 mRNA levels in colorectal cancer cell lines. TDP1 and TOP1 protein levels correlate.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

Requirement of ARFs for ATXR2 function.

PHF10 acts as a transcriptional repressor.

CDCP1 localizes to intracellular vesicles containing lipid rafts.

Incorporation of the TSTA system into a single plasmid.

Neutralization of CSF1 and Ad5-HRG treatment.

Three-day biodistribution of 125I-labeled chTNT-3 administered at (A) 1, 2, or 3 days after pretreatment with VP-16 (30 mg/kg) in MAD109 bearing BALB/c.

Ki-67 expression in M31- and H3-treated tumors (A) and respective Ki-67 labeling indices in the two groups of tumors (B). Ki-67 expression in M31- and.

Chromatin state mapping pinpoints PAX3–FOXO1 (P3F) in active enhancers

Pirh2 represses p73-dependent transactivation.

CCA1 Binds the Promoter of GI in Vitro and in Vivo.

Osteoactivin expression is required for the invasive phenotype of in vivo selected bone metastatic 4T1 breast cancer cells. Osteoactivin expression is.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

ROS Generation, Defense Gene Expression, and Histone Acetylation Levels in the HDT701 OX and RNAi Plants.(A) ROS level in HDT701 RNAi plants after flg22.

HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT signaling. HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT.

A, Proportion for different in silico predictions at the RNA level.

Establishment of HeLa/rtTAA/TRE-N1-IC cell line.

RRP1B interacts with TRIM28 and HP1α at regions associated with H3K9me3 and decreased gene expression. RRP1B interacts with TRIM28 and HP1α at regions.

H3K36me3 is not essential for LEDGF and MLL target gene occupancy.

A, indicated model systems were cultured in media containing complete serum, harvested, and lysed; total protein was separated by SDS-PAGE, transferred.

Mutant TERT promoter displays active histone marks and distinct long-range interactions: A, cell lines that were used in the study with their origin and.

Schematic representation of the HPV genome, highlighting the regions important in PCR-based HPV analysis. Schematic representation of the HPV genome, highlighting.

Presentation transcript:

Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. A, Schematic representation of the genomic structure of the Tβ4 gene for ChIP assay. Boxes denote the exons of Tβ4. The three double-headed arrows indicate the regions examined by ChIP assay. B, Histone modification status in HuH7-F0 and HuH7-F12 cells. ChIP assay was conducted by using antibodies against active (H3K4me3 and H3K27ac) and repressive (H3K9me3 and H3K27me3) histone remarks at the three regions CP1, CP2, and CP3. Representative images and semiquantification measurements are displayed. C, Quantitative ChIP analysis of Tβ4 at the region CP2 in HuH7-F0, HuH7-F6, and HuH7-F12 cells. Bars are the mean ± SE. *, P < 0.01 by ANOVA with Dunnett post hoc test. D, Immunoblots of H3K4me3 and H3K27ac. Yoshiteru Ohata et al. Mol Cancer Ther 2017;16:1155-1165 ©2017 by American Association for Cancer Research