Figure 1. CONSORT diagram.

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Figure 1. CONSORT diagram. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Ann Oncol, Volume 30, Issue 7, 02 May 2019, Pages 1088–1095, https://doi.org/10.1093/annonc/mdz139 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. Clinical outcome of patients in the cfDNA cohort (N = 493) Figure 2. Clinical outcome of patients in the cfDNA cohort (N = 493). Progression-free survival as stratified according ... Figure 2. Clinical outcome of patients in the cfDNA cohort (N = 493). Progression-free survival as stratified according to total circulating cell-free DNA (cfDNA) below and above upper limit of normal (ULN) (A) and quartiles (B). Overall survival as stratified according to cfDNA below and above ULN (C) and quartiles (D). CI, confidence interval. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Ann Oncol, Volume 30, Issue 7, 02 May 2019, Pages 1088–1095, https://doi.org/10.1093/annonc/mdz139 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. Modelling total circulating cell-free DNA levels as a function of 16 predefined variables. Linear and ... Figure 3. Modelling total circulating cell-free DNA levels as a function of 16 predefined variables. Linear and logistic regression models were established to investigate relationships between cfDNA levels and 16 predefined variables belonging to the domains of tumor burden, tumor characteristics and host characteristics of patients in the cfDNA, tissue and IL-6 cohort (N = 364). Variables of tumor burden (N = 3) include resection status of primary tumor, number of metastatic sites and sum of longest tumor diameters (SLD) by RECIST; tumor characteristics (N = 8) include location of primary tumor, tumor RAS and BRAF mutation status, time of metastases, and metastatic involvement of the liver, lung, lymph nodes and peritoneum; host characteristics (N = 5) include age, gender, body mass index (BMI), WHO performance status and systemic inflammatory response (SIR) as reflected by serum level of interleukin 6 (IL-6) (A). Collinearity between explanatory variables used in the models is illustrated using a heatmap. Strength of associations was evaluated as –log10 P values for each variable combination. Fisher's exact test was used for categorical variables, Pearson correlation for continuous variables and Wilcoxon signed-rank test for the combination of categorical and continuous variables (B). The multivariate logistic regression model identified increasing SLD by RECIST, the presence of liver metastases and SIR as measured by IL-6 to significantly account for the likelihood of having cfDNA above ULN (C). OR, odds ratio. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Ann Oncol, Volume 30, Issue 7, 02 May 2019, Pages 1088–1095, https://doi.org/10.1093/annonc/mdz139 The content of this slide may be subject to copyright: please see the slide notes for details.