The ACIN1 Gene is Hypermethylated in Early Stage Lung Adenocarcinoma

Slides:

Advertisements

Similar presentations

Epigenetics Lab December 1, 2008 Goals of today’s lab: 1.Understand the basic molecular techniques used in the lab to study epigenetic silencing in cancer.

Advertisements

Primary Small Cell Carcinoma of the Esophagus Jima Lv, MD, Jun Liang, MD, Jinwan Wang, MD, Luhua Wang, MD, Jie He, MD, Zefen Xiao, MD, Weibo Yin, MD Journal.

Clinicopathologic Characteristics of the EGFR Gene Mutation in Non–small Cell Lung Cancer Anne S. Tsao, MD, Xi Ming Tang, MD, PhD, Bradley Sabloff, MD,

Carcinoma NOS is a Common Histologic Diagnosis and is Increasing in Proportion Among Non-small Cell Lung Cancer Histologies Sai-Hong Ignatius Ou, MD,

Significance of the Number of Positive Lymph Nodes in Resected Non-small Cell Lung Cancer Takayuki Fukui, MD, Shoichi Mori, MD, Kohei Yokoi, MD, Tetsuya.

Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling Baoping Cao, MD, PhD, Weili Yang,

Promoter Methylation and Differential Expression of π-Class Glutathione S-Transferase in Endometrial Carcinoma Queeny K.Y. Chan, Ui-Soon Khoo, Kelvin.

Extensive but hemiallelic methylation of the hMLH1 promoter region in early-onset sporadic colon cancers with microsatellite instability Yasuyuki Miyakura,

Volume 133, Issue 6, Pages (December 2007)

Genome-Wide Identification and Validation of a Novel Methylation Biomarker, SDC2, for Blood-Based Detection of Colorectal Cancer TaeJeong Oh, Nayoung.

ITPKA Gene Body Methylation Regulates Gene Expression and Serves as an Early Diagnostic Marker in Lung and Other Cancers Yi-Wei Wang, PhD, Xiaotu Ma,

Detection of EGFR Mutation Status in Lung Adenocarcinoma Specimens with Different Proportions of Tumor Cells Using Two Methods of Differential Sensitivity

Prognostic Significance of TAZ Expression in Resected Non-Small Cell Lung Cancer Mian Xie, MD, PhD, Li Zhang, MD, Chao-Sheng He, MD, Jin-Hui Hou, MD,

Differential Diagnosis of Primary Versus Metastatic Pulmonary Adenocarcinomas Using Gene Mutation Analyses: A Case Report Yoshimasa Nakazato, MD, Ryota.

Bronchioloalveolar Carcinoma (Lepidic Growth) Component Is a More Useful Prognostic Factor than Lymph Node Metastasis Yoichi Anami, MD, Tatsuo Iijima,

Qing Cheng, Cheng Cheng, Kristine R. Crews, Raul C

Yih-Leong Chang, MD, Chen-Tu Wu, MD, Yung-Chie Lee, MD, PhD

GATA2 is Epigenetically Repressed in Human and Mouse Lung Tumors and Is Not Requisite for Survival of KRAS Mutant Lung Cancer Mathewos Tessema, PhD,

Can Aberrant Promoter Hypermethylation of CpG Islands Predict the Clinical Outcome of Non-Small Cell Lung Cancer After Curative Resection? Young Tae.

Long-Lasting Complete Remission with Tyrosine Kinase Inhibitor in Bronchioloalveolar Carcinoma with a so far Unknown EGFR Mutation Vienna Ludovini, PhD,

Tetsuya Mitsudomi, MD, Hirohito Tada, MD Journal of Thoracic Oncology

Electronic Updates for JTO Readers

Membrane Carbonic Anhydrase IX Expression and Relapse Risk in Resected Stage I–II Non–Small-Cell Lung Cancer David J. Stewart, MD, Maria I. Nunez, MD,

S. Sesselmann, M. D. , S. Söder, M. D. , R. Voigt, J. Haag, Ph. D. , S

Samuel J. Wang, MD, PhD, C David Fuller, MD, Charles R. Thomas, MD

Volume 42, Issue 4, Pages (April 2005)

Frank Detterbeck, MD Journal of Thoracic Oncology

Clinicopathologic Characteristics of the EGFR Gene Mutation in Non–small Cell Lung Cancer Anne S. Tsao, MD, Xi Ming Tang, MD, PhD, Bradley Sabloff, MD,

Kelsie L. Thu, BSc, Raj Chari, PhD, William W

Tumor Cell Content for Selection of Molecular Techniques for T790M EGFR Mutation Detection in Non-small Cell Lung Cancer Nathalie Prim, Elisabeth Quoix,

Volume 121, Issue 6, Pages (December 2001)

Mucinous Adenocarcinoma of the Thymus: A Case Report

G-Protein Coupled Receptor Family C, Group 5, Member A (gprc5a) Expression Is Decreased in the Adjacent Field and Normal Bronchial Epithelia of Patients.

Custom-Designed MLPA Using Multiple Short Synthetic Probes

Epidermal Growth Factor Receptor Mutation and p53 Overexpression during the Multistage Progression of Small Adenocarcinoma of the Lung Seol Bong Yoo,

Intratumoral Lymphatic Vessel Involvement is an Invasive Indicator of Completely Resected Pathologic Stage I Non-small Cell Lung Cancer Masahiko Harada,

Jeffrey Bradley, MD, Issam El Naqa, PhD Journal of Thoracic Oncology

Hypermethylation of estrogen receptor-α gene in atheromatosis patients and its correlation with homocysteine Yu-Shan Huang, Yan-Fang Zhi, Shu-Ren Wang

Effects of Chemotherapy on Survival of Elderly Patients with Small-Cell Lung Cancer: Analysis of the SEER-Medicare Database Laura C. Caprario, MD, MS,

A Single Institution-Based Retrospective Study of Surgically Treated Bronchioloalveolar Adenocarcinoma of the Lung: Clinicopathologic Analysis, Molecular.

MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies Santosh Patnaik, MD, PhD, Reema.

A Comprehensive Analysis of p16 Expression, Gene Status, and Promoter Hypermethylation In Surgically Resected Non-small Cell Lung Carcinomas William.

Survival Differences by Gender for Resected Non-small Cell Lung Cancer: A Retrospective Analysis of 12,509 Cases in a Japanese Lung Cancer Registry Study

Volume 122, Issue 4, Pages (April 2002)

Appropriateness of Surgical Approach in Black Patients with Lung Cancer—15 Years Later, Little Has Changed Emanuela Taioli, MD, PhD, Raja Flores, MD

Positron Emission Tomography 18F-Fluorodeoxyglucose Uptake and Prognosis in Patients with Surgically Treated, Stage I Non-small Cell Lung Cancer: A Systematic.

Vamsidhar Velcheti, MD Journal of Thoracic Oncology

Remco van Doorn, Willem H. Zoutman, Nelleke A. Gruis

An Unmet Need in the WHO 2015 Biopsy Classification: Poorly Differentiated NSCCs with Positive Neuroendocrine Markers Jules L. Derks, MD Journal of.

Aglaya G. Iyevleva, MD, PhD Journal of Thoracic Oncology

Use of MicroRNA Expression Levels to Predict Outcomes in Resected Stage I Non- small Cell Lung Cancer Eric Duncavage, MD, Boone Goodgame, MD, Ananth Sezhiyan,

Genome–Wide Hypomethylation and Specific Tumor-Related Gene Hypermethylation are Associated with Esophageal Squamous Cell Carcinoma Outcome Sojun Hoshimoto,

Histological Scoring for Small Lung Adenocarcinomas 2 cm or Less in Diameter: A Reliable Prognostic Indicator Akiko Miyagi Maeshima, MD, Naobumi Tochigi,

The human GPR109A promoter is methylated and GPR109A expression is silenced in human colon carcinoma cells. The human GPR109A promoter is methylated and.

Imprinting of Human GRB10 and Its Mutations in Two Patients with Russell-Silver Syndrome Hiroshi Yoshihashi, Katsuhiro Maeyama, Rika Kosaki, Tsutomu.

Validation of the Proposed International Association for the Study of Lung Cancer Non- small Cell Lung Cancer Staging System Revisions for Advanced Bronchioloalveolar.

Volume 131, Issue 6, Pages (December 2006)

Functional Modulation of IGF-Binding Protein-3 Expression in Melanoma

Dan J. Raz, MD, Jason A. Zell, DO, MPH, Anthony N

Activation of MET by Gene Amplification or by Splice Mutations Deleting the Juxtamembrane Domain in Primary Resected Lung Cancers Ryoichi Onozato, MD,

Neuronatin Expression and Its Clinicopathological Significance in Pulmonary Non-small Cell Carcinoma Teruhito Uchihara, MD, Chigusa Okubo, MS, Ryota.

Wedge Resection for Non-small Cell Lung Cancer in Patients with Pulmonary Insufficiency: Prospective Ten-Year Survival John P. Griffin, MD, Charles E.

Significance of the Number of Positive Lymph Nodes in Resected Non-small Cell Lung Cancer Takayuki Fukui, MD, Shoichi Mori, MD, Kohei Yokoi, MD, Tetsuya.

Prognostic implication of aberrant promoter hypermethylation of CpG islands in adenocarcinoma of the lung Young Tae Kim, MD, PhD, Sun Jung Park, MS,

Methylation status of IGFBPL1 in human breast cancer.

Analysis of Differentially Expressed Genes in Neuroendocrine Carcinomas of the Lung Chigusa Okubo, MS, Yuko Minami, MD, Ryota Tanaka, MD, Teruhito Uchihara,

CpG Methylation Analysis—Current Status of Clinical Assays and Potential Applications in Molecular Diagnostics Antonia R. Sepulveda, Dan Jones, Shuji.

Rogerio C. Lilenbaum, MD, John Cashy, PhD, Thomas A

Outcome after Pulmonary Metastasectomy: Analysis of 5 Years Consecutive Surgical Resections 2002–2006 Kåre Hornbech, MD, Jesper Ravn, MD, Daniel Andreas.

Qing Cheng, Cheng Cheng, Kristine R. Crews, Raul C

Presentation transcript:

The ACIN1 Gene is Hypermethylated in Early Stage Lung Adenocarcinoma Yujian Shu, MD, Tatsuo Iijima, MD, Weihong Sun, MD, Junko Kano, MD, Tadashi Ishiyama, MS, Chigusa Okubo, MS, Yoichi Anami, MD, Ryota Tanaka, MD, Shimao Fukai, MD, Masayuki Noguchi, MD Journal of Thoracic Oncology Volume 1, Issue 2, Pages 160-167 (February 2006) DOI: 10.1016/S1556-0864(15)31532-X Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 1. a: Phase-contrast microphotograph of PL16T which is a immortalized cell line derived from atypical adenomatous hyperplasia. (original magnification x100) b: Phase-contrast microphotograph of PL16B which is a immortalized cell line derived from the resected end of bronchus of the same patient. (original magnification x100) c: Histological feature of small-sized adenocarcinoma with bronchioloalveolar carcinoma component. (case 5 in table 2) (original magnification x4) d: Histological feature of small-sized adenocarcinoma without bronchioloalveolar carcinoma component. (case 24 in table 2) (original magnification x4) Journal of Thoracic Oncology 2006 1, 160-167DOI: (10.1016/S1556-0864(15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 2. Genome structure of the ACIN1 gene and the locations of CpG islands (full black boxes). The position of the DNA fragment isolated by MCA-SSH is indicated by the open box. C denotes the cytosine residues at the 5′: end of the isolated DNA fragment, with unknown methylation status. During sodium bisulfite DNA modification, unmethylated cytosine residues are converted to thymine, whereas methylated cytosine residues are retained as cytosine at the CpG site. After bisulfite DNA modification, the DNA fragments were amplified by PCR using appropriate primers and their sequences were determined with an automated sequencer. Journal of Thoracic Oncology 2006 1, 160-167DOI: (10.1016/S1556-0864(15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 3. Expression of the ACIN1 gene. Expression levels were analyzed by quantitative RT-PCR in the pair of cell lines (PL16T and PL16B), in three surgical specimens of lung adenocarcinoma with BAC component (case numbers 11, 13, 21; see table 2) and in three specimens of adenocarcinoma without BAC component (case numbers 25, 26, 27; see table 2) together with their normal adjacent tissues. T with BAC: adenocarcinoma with bronchioloalveolar carcinoma component; T without BAC: adenocarcinoma without bronchioloalveolar carcinoma component. Journal of Thoracic Oncology 2006 1, 160-167DOI: (10.1016/S1556-0864(15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions