Impaired sperm aggregation in Adam2 and Adam3 null mice Cecil Han, M.Sc., Jun Tae Kwon, M.Sc., Inju Park, M.Sc., Boyeon Lee, M.Sc., Sora Jin, M.Sc., Heejin Choi, M.Sc., Chunghee Cho, Ph.D.  Fertility and Sterility  Volume 93, Issue 8, Pages 2754-2756 (May 2010) DOI: 10.1016/j.fertnstert.2010.03.013 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Defective sperm aggregation of Adam2-/- or Adam3-/- sperm. Wild-type (WT), Adam2-/-, and Adam3-/- sperm were observed under a light microscope after incubation at 37°C for 10 minutes in M16 medium. Typical sperm are magnified in the insets. Bars = 100 μm. (B) The levels of sperm aggregation and motility were represented as a mean percentage of the control (WT) ± SD. Experiments were repeated three times (each time with sperm from a different male mouse). (C) The levels of sperm aggregation after incubation for 0, 10, 30, and 60 minutes were presented as a mean percentage of the control (WT at 30 minutes). After 60 minutes of incubation, sperm were subjected to pipetting and sperm grouping was monitored further. 2KO = Adam2-/-; 3KO = Adam3-/-. Fertility and Sterility 2010 93, 2754-2756DOI: (10.1016/j.fertnstert.2010.03.013) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions