Basic Microscopy SC.912.L.14.4 - Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom.

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Basic Microscopy SC.912.L.14.4 - Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: The Far Side by Gary Larson

Compound Light Microscope Compound microscopes have two sets of lenses for magnification. ocular lens = Lens closer to the eye (magnifying power of 10x). objective lens - Lenses closer to the object being viewed (Most light microscopes used in biology have three or four objective lenses). Produce a dark image against brighter, backlit background. Provide a 2-D image of thinly sliced specimens on a slide. Commonly used to view stained cells. Image: Magnifying lamp use to look for part defects, US Navy; Compound light microscope, Moisey From the Virtual Microbiology Classroom on ScienceProfOnline.com

Stereoscopic (Dissecting) Microscope Used for viewing live specimens or three-dimensional objects too large or thick to be accommodated by compound microscopes. Not as powerful as compound microscopes

Electron Microscopes Used to observe VERY small objects: viruses, DNA, parts of cells Uses beams of electrons rather than light Electron beam wavelengths are shorter than light wavelengths, so better resolving power. Transmission Electron Microscope (TEM): 2-D image, see through the specimen Scanning Electron Microscope (SEM): 3-D image, see surface of the specimen Images: Poliovirus, taken with TEM, PHIL #1875, Blood cells, taken with SEM, National Cancer Institute; Scanning electron microscope, Geological Survey of Israel laboratory. From the Virtual Microbiology Classroom on ScienceProfOnline.com

What is the difference between electron microscope and light (optical) microscope? • Electron microscope uses an electron beam, while an optical microscope uses a light beam. • The maximum magnification of the optical microscope is about 2000x, where the maximum magnification of the electron microscope is about 10,000,000x. Human eye used to see these Light Microscope used to see these. Electron Microscope used to see these