Ultrastructural localization of NKCC2 protein in the TAL and macula densa (MD). Ultrastructural localization of NKCC2 protein in the TAL and macula densa.

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Ultrastructural localization of NKCC2 protein in the TAL and macula densa (MD). Ultrastructural localization of NKCC2 protein in the TAL and macula densa (MD). (A) Immunoelectron microscopy of NKCC2 in the TAL. NKCC2 labeling is associated with apical plasma membrane (arrows) and small intracellular vesicles (arrowheads) of TAL cells. Both smooth-surfaced cells (left) and rough-surfaced cells (right) are labeled, with greater labeling of intracellular vesicles in smooth-surfaced cells. (B) Immunogold localization of NKCC2 in ions of MD. Abundant NKCC2 labeling is associated with apical plasma membrane (arrows) of MD cells and TAL cells. Inset: overview showing MD cells and TAL cells. Regions indicated by MD are shown at higher power in main panel from adjacent section. Original magnification, ×3500 in B; ×44,000 in B inset. Reprinted from reference 4, with permission. David B. Mount CJASN 2014;9:1974-1986 ©2014 by American Society of Nephrology