Effects of Dextran Molecular Weight on Red Blood Cell Aggregation

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Presentation transcript:

Effects of Dextran Molecular Weight on Red Blood Cell Aggregation Björn Neu, Rosalinda Wenby, Herbert J. Meiselman Biophysical Journal Volume 95, Issue 6, Pages 3059-3065 (September 2008) DOI: 10.1529/biophysj.108.130328 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 Viscosity concentration results for dextran molecular mass fractions used in this study. Data were obtained at 25°C using an automated tube viscometer. Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 70 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 250 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 500 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 2 MDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 28 MDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 7 Calculated dependence of RBC aggregation on molecular mass at different suspending phase polymer concentrations. (A) M value and (B) M1 value. Data points were computed based on the experimental results shown in Figs. 2–6. Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 8 Calculated dependence of RBC aggregation on molecular mass at different suspending phase polymer concentrations. (A) M value and (B) M1 value. Data points were computed based on the experimental results shown in Figs. 2–6 (see Materials and Methods). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 9 Theoretical dependence of depletion energy on polymer molecular mass for a suspending phase polymer concentrations c2 of 0.5g/dL and penetration depth p of 0 and 5nm, respectively (see text for details). Biophysical Journal 2008 95, 3059-3065DOI: (10.1529/biophysj.108.130328) Copyright © 2008 The Biophysical Society Terms and Conditions