RT-PCR analysis of Wnt genes in PLC-PRF-5 and HLF cell lines.

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RT-PCR analysis of Wnt genes in PLC-PRF-5 and HLF cell lines. RT-PCR analysis of Wnt genes in PLC-PRF-5 and HLF cell lines. Total RNA was isolated from PLC-PRF-5, HLF, MCF-7, SKVO3, PANC-1, BxPC3, and Jurkat cell lines or human placenta, and cDNA was synthesized using reverse transcriptase (RT, +). As a negative control, the same RNAs were incubated in the absence of reverse transcriptase (−). PCR was then done with specific primers. Only includes results for the Wnts in which at least one of the cell lines was positive [gene name (right) along with the size of the amplicon]. The positive controls used were human placenta for Wnt2B, Wnt3, and Wnt4; SKVO3 cells for Wnt5A; PANC-1 cells for Wnt5B; MCF-7 cells for Wnt7B; BxPC3 cells for Wnt10A and Jurkat cells for Wnt10B. Human β-actin was used as a constitutively expressed marker to assess relative amounts of RNA in each sample. Mariana I. Capurro et al. Cancer Res 2005;65:6245-6254 ©2005 by American Association for Cancer Research