HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential.

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HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. Indicated HNSCC and NSCLC cells cultured in poly(HEMA)-coated plates (PCP) and in ultralow attached plates (UAP) were treated with hIgG1 (25 μg/mL) or IMC-cixutumumab (25 μg/mL) for 3 (A, C, and D) or 5 days (B) in the presence of FBS or for 6 hours in the absence of FBS and then stimulated with 10% FBS for 30 minutes (A, bottom). A, representative morphologies of LN686 and OSC19 cells (Con, control; Cixu, cixutumumab). A (bottom) and D, Western blot was done for the indicated proteins. B and C, cell viabilities were measured by using MTS assay and were determined as percentages of each control groups. Independent experiments were repeated 3 times. Bars represent mean ± SD (n = 6); *, P < 0.05 and **, P < 0.01. The statistical significance was determined by using Student t test. Dong Hoon Shin et al. Mol Cancer Ther 2011;10:2437-2448 ©2011 by American Association for Cancer Research