A and B, mRNA expression relative to ERα (in%) for ZNF423 (A) and BRCA1 (B) for LCLs of known genotypes for variant (V) and WT chromosome 16 ZNF423 SNPs (3 cell lines each), incubated for 24 hours with E2 concentrations that varied from 0.00001 to 0.01 nmol... A and B, mRNA expression relative to ERα (in%) for ZNF423 (A) and BRCA1 (B) for LCLs of known genotypes for variant (V) and WT chromosome 16 ZNF423 SNPs (3 cell lines each), incubated for 24 hours with E2 concentrations that varied from 0.00001 to 0.01 nmol/L. Error bars represent SEM. C, ZNF423 mRNA expression relative to baseline in Hs578T cells after ZNF423 overexpression (OE; top left) or ZNF423 knockdown (KD; top right) together with corresponding BRCA1 transcriptional activity measured by luciferase activity at 48 hours (bottom). Error bars represent SEM. D, ChIP assay using human Hs578T cells stably transfected with ERα showing binding of ZNF423 to a region containing a ZNF423 consensus binding sequence located 1,735 to 1,518 bp from the ATG in BRCA1. James N. Ingle et al. Cancer Discovery 2013;3:812-825 ©2013 by American Association for Cancer Research