SlRd2 expression confers LiCl resistance in yeast and its dimerization is required for its biological function. SlRd2 expression confers LiCl resistance.

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SlRd2 expression confers LiCl resistance in yeast and its dimerization is required for its biological function. SlRd2 expression confers LiCl resistance in yeast and its dimerization is required for its biological function. A, BY4741 yeast strain expressing SlRd2 (cloned into p426GPD) was subjected to different stress conditions. As a control, BY4741 was transformed with the EV. Both SlRd2 and EV lines were grown in liquid culture and spotted on specific medium at different dilutions. Overexpression of SlRd2 showed a higher growth than EV in response to LiCl (300 mM). B, Overexpression of SlRd2Δdim showed the same growth rate than EV line in response to LiCl. The experiment was performed as described in (A). C, Expression of SlRd2 and SlRd2Δdim proteins in yeast were confirmed by immunoblotting using anti-SlRd2 antibodies. Emilio Gutiérrez-Beltrán et al. Plant Physiol. 2016;173:836-852 ©2017 by American Society of Plant Biologists