Evidence for a lipid transfer protein as the major allergen of apricot Elide A. Pastorello, MDa, Francesco Purello D’Ambrosio, MDb, Valerio Pravettoni, MDa, Laura Farioli, BScc, Gabriella Giuffrida, BScd, Mara Monza, BSca, Raffaella Ansaloni, MDe, Donatella Fortunato, BScd, Elisabetta Scibola, MDa, Federica Rivolta, MDa, Cristoforo Incorvaia, MDa, Anders Bengtsson, BScf, Amedeo Conti, BScd, Claudio Ortolani, MDe Journal of Allergy and Clinical Immunology Volume 105, Issue 2, Pages 371-377 (February 2000) DOI: 10.1016/S0091-6749(00)90090-3 Copyright © 2000 Mosby, Inc. Terms and Conditions
Fig. 1 IgE immunoblots of apricot extract in 30 patients with OAS. Patients 1 to 16 had birch pollinosis, and patients 17 to 30 did not have birch pollinosis. Journal of Allergy and Clinical Immunology 2000 105, 371-377DOI: (10.1016/S0091-6749(00)90090-3) Copyright © 2000 Mosby, Inc. Terms and Conditions
Fig. 2 Cation exchange of apricot extract fractionated over the Resource-S HPLC column (A) . Gel filtration of the peak obtained in cation-exchange chromatography (B) . Journal of Allergy and Clinical Immunology 2000 105, 371-377DOI: (10.1016/S0091-6749(00)90090-3) Copyright © 2000 Mosby, Inc. Terms and Conditions
Fig. 3 SDS-PAGE of total apricot extract and the 9-kd purified protein stained with Coomassie Brilliant Blue and IgE immunoblotting of the 9-kd purified protein. Journal of Allergy and Clinical Immunology 2000 105, 371-377DOI: (10.1016/S0091-6749(00)90090-3) Copyright © 2000 Mosby, Inc. Terms and Conditions
Fig. 4 Blotting inhibition of crude apricot extract by the peach 9-kd purified protein. Journal of Allergy and Clinical Immunology 2000 105, 371-377DOI: (10.1016/S0091-6749(00)90090-3) Copyright © 2000 Mosby, Inc. Terms and Conditions