GPR55 regulates γδT cell egress from PP and homing of gut-tropic CD8 T cells to the small intestine. GPR55 regulates γδT cell egress from PP and homing.

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GPR55 regulates γδT cell egress from PP and homing of gut-tropic CD8 T cells to the small intestine. GPR55 regulates γδT cell egress from PP and homing of gut-tropic CD8 T cells to the small intestine. (A) Frequency of CD8αα γδT cells in PPs, mLN, and peripheral blood lymphocytes (PBLs) of GPR55 Het (DMSO, n = 5; Indo, n = 8) and KO (DMSO, n = 4; Indo, n = 8) mice after treatment with carrier (DMSO) or Indo for 4 hours. (B) Ratio of CD45.2 GPR55 KO over CD45.1/2 GPR55 WT CD8αα γδT cells in the indicated tissues of mixed BM chimeras 4 hours after treatment with DMSO or Indo (n = 4 in each group). (C) Percentage (left) or numbers (right) of CD8αα γδT cells in the indicated GPR55 Het and KO mouse tissues after treatment for 4 hours with Indo and FTY720. Left: GPR55 Het, n = 9; GPR55 KO, n = 8. Right: GPR55 Het, n = 6; GPR55 KO, n = 9. (D) Migration of the indicated cell types from PPs of control and GPR55 KO mice to nil, CXCL12, or CXCL12 and 20:4 LPI in Transwell assays. Each experiment was performed in duplicate and repeated twice. (E) Expression of Ccr9 and Gpr55 mRNA on T cells cultured for the indicated days in the presence of anti-CD3 and anti-CD28 and with IL-2 (100 U/ml) from day 3 and in the absence or presence of retinoic acid (RA), plotted relative to Hprt (n = 4 in each group from two independent experiments). Bars indicate mean ± SEM. (F) Homing of CD8 T cell cultured with RA as in (E) to the spleen, small intestine, and blood, shown as the ratio of CD45.2 GPR55 KO cells to CD45.1 WT cells, 1 day after transfer. Data (n = 4 in each group) are representative of two independent experiments. (G) Homing of cells cultured as in (E) and transduced with empty or GPR55-containing vector, plotted as the ratio of transduced (Thy1.1 reporter–positive) to untransduced (Thy1.1 reporter–negative) cells of each type, 1 day after transfer. “Injected cells” in (F) and (G) indicates the ratio at the time of injection. Data (n = 3 in each group) are representative of two independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05, n.s. P > 0.05 by Student’s t test (A to C, F, and G) or one-way ANOVA (E). Hayakazu Sumida et al. Sci. Immunol. 2017;2:eaao1135 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works