CIKs Can Be Phosphorylated by BAM1/2 and RPK2.

Slides:

Advertisements

Similar presentations

Volume 41, Issue 6, Pages (March 2011)

Advertisements

Large Hepatitis Delta Antigen Modulates Transforming Growth Factor-β Signaling Cascades: Implication of Hepatitis Delta Virus–Induced Liver Fibrosis

IP-MS identifies FAM49B interacting protein Rac.

Volume 49, Issue 6, Pages (March 2013)

Volume 13, Issue 2, Pages (January 2004)

Autoinhibition of c-Abl

Phosphorylation of NF-κB p65 by PKA Stimulates Transcriptional Activity by Promoting a Novel Bivalent Interaction with the Coactivator CBP/p300 Haihong.

H-NS2 protein interacts with Lon and H-NS protein in vitro.

Histone deacetylase 3 associates with and represses the transcription factor GATA-2 by Yukiyasu Ozawa, Masayuki Towatari, Shinobu Tsuzuki, Fumihiko Hayakawa,

by Yuka Nagata, Eisuke Nishida, and Kazuo Todokoro

Activating and Silencing the Mitotic Checkpoint through CENP-E-Dependent Activation/Inactivation of BubR1 Yinghui Mao, Ariane Abrieu, Don W. Cleveland

A Conversation across Generations: Soma-Germ Cell Crosstalk in Plants

Phosphorylation of a tyrosine residue in mGluR1a-CT1 by Fyn.

Tapetal PCD Occurs Prematurely in the myb80 Mutant

Hexin Tan, Wanqi Liang, Jianping Hu, Dabing Zhang Developmental Cell

The ΔrlmA mutant strain has impaired CWI pathway activation.

Volume 34, Issue 2, Pages (July 2015)

Selective Degradation of Ubiquitinated Sic1 by Purified 26S Proteasome Yields Active S Phase Cyclin-Cdk Rati Verma, Hayes McDonald, John R Yates, Raymond.

NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus. NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus.

MAPKKK5-induced cell death is independent of Ser-289 phosphorylation.

MED12 interacts with TDRD3 in a CARM1-dependent fashion.

PKA phosphorylates Thr63 and Ser692 to increase HIF-1α stability.

PKA interacts with HIF-1α.

EXS, a Putative LRR Receptor Kinase, Regulates Male Germline Cell Number and Tapetal Identity and Promotes Seed Development in Arabidopsis Claudia Canales,

Ligand-Independent Recruitment of SRC-1 to Estrogen Receptor β through Phosphorylation of Activation Function AF-1 André Tremblay, Gilles B Tremblay,

Volume 12, Issue 8, Pages (August 2015)

Shijiao Huang, Danming Tang, Yanzhuang Wang Developmental Cell

Per Stehmeier, Stefan Muller Molecular Cell

Volume 9, Issue 9, Pages (September 2016)

Volume 13, Issue 2, Pages (January 2004)

Specificity of methylation mediated by the COPR5–PRMT5‐containing complex. Specificity of methylation mediated by the COPR5–PRMT5‐containing complex. (A)

FAK-Mediated Src Phosphorylation of Endophilin A2 Inhibits Endocytosis of MT1-MMP and Promotes ECM Degradation Xiaoyang Wu, Boyi Gan, Youngdong Yoo,

Volume 25, Issue 18, Pages (September 2015)

Volume 57, Issue 3, Pages (February 2015)

Regulation of RECQ4–MCM10 interaction by CDK phosphorylation.

Volume 21, Issue 3, Pages (September 2011)

Hua Gao, Yue Sun, Yalan Wu, Bing Luan, Yaya Wang, Bin Qu, Gang Pei

SUR-8, a Conserved Ras-Binding Protein with Leucine-Rich Repeats, Positively Regulates Ras-Mediated Signaling in C. elegans Derek S Sieburth, Qun Sun,

Regulation of Yeast mRNA 3′ End Processing by Phosphorylation

NF-κB Is Required for UV-Induced JNK Activation via Induction of PKCδ

Volume 13, Issue 3, Pages (February 2004)

Ub-binding ability of ERα.

Characterization of the ERα-UBS.

LINT-25 and LAP2α interact in vitro.

GOLPH3 interacts with COG complex proteins.

DAPK interacts with HSF1 in vivo and in vitro.

Volume 10, Issue 4, Pages (April 2017)

Volume 15, Issue 3, Pages (March 2014)

Β-Catenin regulation of AChR clustering requires interaction with α-catenin. β-Catenin regulation of AChR clustering requires interaction with α-catenin.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Mapping the Pirh2 and p73 interaction sites.

MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. (A) Y2H.

Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N

βC1 interferes with the interaction between WRKY20 and ERF-ORA59

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

BAF57 binds AR DBD/hinge region.

Volume 13, Issue 14, Pages (July 2003)

PVT1 binds to the FOXM1 protein and increases its protein level by enhancing its stability. PVT1 binds to the FOXM1 protein and increases its protein level.

PKA phosphorylates p47 at a novel site that regulates dendritic complexity. PKA phosphorylates p47 at a novel site that regulates dendritic complexity.

Gα12 and Gα13 Interact with Ser/Thr Protein Phosphatase Type 5 and Stimulate Its Phosphatase Activity Yoshiaki Yamaguchi, Hironori Katoh, Kazutoshi Mori,

The LIN-2/LIN-7/LIN-10 Complex Mediates Basolateral Membrane Localization of the C. elegans EGF Receptor LET-23 in Vulval Epithelial Cells Susan M Kaech,

Volume 11, Issue 2, Pages (February 2018)

SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of.

MLK4 Is Required for H2A.Z Deposition and H4 Acetylation.

Rapamycin-Dependent Interaction between TOR-FRB Domain and Hs FKBP12

EAR1 Represses ABA Signaling.

RRC1 Interacts with phyB and Colocalizes in Nuclear Photobodies.

MED25 and JAZ7 Compete to Interact with MYC2.

Design of lipid-binding deficient mutants of α-synuclein.

MTB1 Interacts with MYC2 and Disrupts MED25-MYC2 Interaction.

Presentation transcript:

CIKs Can Be Phosphorylated by BAM1/2 and RPK2. CIKs Can Be Phosphorylated by BAM1/2 and RPK2. (A) and (C) CIK1KD and CIK3KD interact with BAM1CD, BAM2CD, and RPK2KD in vitro. CIK1KD-His (A) and CIK3KD-His (C) fusion proteins were incubated with glutathione beads bound with GST, GST-BAM1CD, GST-BAM2CD, or GST-RPK2KD. The pulled-down (PD) proteins were immunoblotted with an α-His antibody (IB, α-His). (B) and (D) In vitro kinase assays showing phosphorylation of CIK1KDKm (B) or CIK3KDKm (D) by BAM1CD and BAM2CD. BAM1CDKm and BAM2CDKm cannot phosphorylate CIK1KDKm and CIK3KDKm. (E) In vitro kinase assays showing phosphorylation of CIK1KDKm by RPK2CD. RPK2CDKm cannot phosphorylate CIK1KDKm. An α-pThr antibody was used to detect the phosphorylation levels. The input proteins were stained with Coomassie Brilliant Blue (CBB). These experiments were repeated three times independently with similar results. (F) CIKs function as coreceptors of BAM1/2 and RPK2 to regulate early anther development. CIKs physically interact with BAM1/2 and RPK2 to ensure specification and maintenance of the parietal cell fate. In the quadruple cik mutant, the primary parietal cell cannot be properly specified because of the altered division of the archesporial cell, which produces MMC-like cells enclosed only by the epidermis. The inner secondary parietal cell and the outer secondary parietal cell cannot be properly specified in some areas of cik anther wall either, resulting in MMC-like cells surrounded by one or two parietal cell layers and the epidermis. RPK2-CIKs and EMS1-SERKs complexes coordinate to determine the inner secondary parietal cell fate. The inner secondary parietal cell only forms the tapetal cell without the function of CIKs or RPK2, whereas it only forms the middle layer cell in ems1 or serk1/2 mutants. Anther stages (St) 2 to 5 are represented. Ar, archesporial cell; E, epidermis; En, endothecium; ML, middle layer; PP, primary parietal cell; PS, primary sporogenous cell; ISP, inner secondary parietal cell; OSP, outer secondary parietal cell; Sp, sporogenous cell; T, tapetum. Yanwei Cui et al. Plant Cell 2018;30:2383-2401 ©2018 by American Society of Plant Biologists