Warm-Up, top of page 8 How does knowing how to use a microscope help me in Living Earth?

Microscope Intro NBpg. 9 EQ: How does this device aid in seeing the microscopic? Page 8 (Q1) A microscope is a device that produces magnified images of structures that are too small to see with an unaided eye. Types of Microscopes: Dissecting microscope produces magnified images by focusing visible light rays. Compound light microscope allows light to pass through the specimen and use two lenses to form an image. Electron microscope uses beams of electrons to produce magnified images.

Microscope Intro NBpg. 9 EQ: How does this device aid in seeing the microscopic? Page 8 (Q1) (Q2) A microscope is a device that produces magnified images of structures that are too small to see with an unaided eye. Types of Microscopes: Dissecting microscope produces magnified images by focusing visible light rays. Compound light microscope allows light to pass through the specimen and use two lenses to form an image. Electron microscope uses beams of electrons to produce magnified images.

Microscopes (Cont.) Important! NBpg. 9 Microscopes (Cont.) Page 8 Sketch: Important! Handle the microscope with care One hand holds the arm and place one hand under the base. Field of View: The area you see when looking into the objective Magnification: Under the field of view, write the Magnification you are looking at. Summary: Answer EQ (Q3) (Q4) 40x

Video: How to Use the Compound Light Microscope https://www.youtube.com/watch?v=lo2aC_m2v yo&t=335s

Bonus Slides There are four bonus slides available after you complete the assignment on the following slide Two slides are of brown hair, and 2 are of gray hair Extra credit to add a 4th field of view and then draw, color and label the 2 hairs side-by-side

Microscope Lab Activity NBpg. 8 under warm-up Microscope Lab Activity The Letters Draw your field of view and label your magnification What did you notice about the letters. What could make them that way? Threads Draw and color your field of view and label your magnification. Which magnification gets you closest to the object? What details do you see at higher magnification? Draw the colors in order from bottom to top. Measurement Draw your field of view and label your magnification at 40x Count the number of Millimeters at 40x. Count the number of mm at 100x. x x x

Microscope Lab Study the microscope labeling sheet, so you are familiar with the microscope’s parts. Instructions Plug in the microscope and turn the switch to the “on” position. Check that the stage is as far down as it can go and the 4X objective lens is in place. Place your prepared sample on the stage and then secure it with the stage clips. Center the sample under the light; if the light isn’t bright enough use the diaphragm to increase the light intensity. Place your eye to the eye piece and then focus the sample by turning the coarse adjustment knob so the stage rises; once you can see the sample well, use the fine adjustment knob to bring the sample fully into focus. Now turn the objective lens to the next (middle) setting making sure it snaps into place. Use only the fine adjustment knob to focus. (Don’t use the coarse adjustment knob, because the slide can break!) Now turn the objective lens to the next (highest) setting making sure it snaps into place. Use only the fine adjustment knob to focus. Don’t use the coarse adjustment knob, because the slide can break!) Draw what you see into your lab page in the “field of view” circle; record your magnification level and answer any questions. When finished, lower the stage all the way down, turn the objective lens to the lowest setting (usually 4X), remove any slides/samples, turn off the microscope and then unplug it. Clean up your work space for the next group that will be using it.

Regular e Low power 40X