Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

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Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. A, Comet image showing significantly greater DNA tail formation in MMR+ M4 cells than in MMR− V2 cells on day 1 and day 2 after 6-TG (3 μm × 24 h) removal. Gels were stained with Hoechst 33342, and pictures were taken under a fluorescence microscope (magnification, ×100). B, tail moment (fraction of DNA in the tail × tail length) analyzed with ImageJ software. Data are calculated from measurement of 50 Comets for each sample. Tao Yan et al. Clin Cancer Res 2003;9:2327-2334 ©2003 by American Association for Cancer Research