Cytoplasmic accumulation of Bach1 in response to heme

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Heme regulates gene expression by triggering Crm1‐dependent nuclear export of Bach1 Cytoplasmic accumulation of Bach1 in response to heme. (A) 293T cells were cotransfected with FLAG‐Bach1 and MafK expression plasmids. At the end of culturing, cells were treated with 10 μM CdCl2 (second row) or hemin (third row) for 4 h. Bach1 and MafK were detected using anti‐FLAG and anti‐MafK antibodies, respectively. Images for Bach1, MafK, and DNA are shown (left to right). (B) Subcellular localization of Bach1 was classified into three categories: C>N, cytoplasmic‐dominant accumulation (gray bar); C=N, roughly equal distribution in cytoplasmic and nuclear compartments (white bar); and C<N, nuclear‐dominant accumulation (black bar). Results of counting 200 cells are shown. (C) Domains of Bach1. BTB and CLS facilitate cytoplasmic accumulation, whereas the basic region facilitates nuclear accumulation (Suzuki et al, 2003). CP motifs are shown with circled numbers. Bach1 derivatives tagged with FLAG at the N‐termini are shown below, with the positions of amino‐acid residues marked. (D, E) 293T cells were transfected with Bach1ΔBTB or Bach1ΔBTBΔC1 expression plasmids and treated with cadmium (second row) or hemin (third row). Images are DNA (right) and Bach1 (left). (F) Subcellular distributions of Bach1ΔBTB or Bach1ΔBTBΔC1 in 293T cells were counted as in (B). Results of counting 200 cells are shown. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 23, Issue: 13, Pages: 2544-2553, First published: 03 June 2004, DOI: (10.1038/sj.emboj.7600248)