Cocultivation of fibroblasts with epithelial cells (EP156T-luc).

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Cocultivation of fibroblasts with epithelial cells (EP156T-luc). Cocultivation of fibroblasts with epithelial cells (EP156T-luc). EP156T-luc cells (1 × 103) were either cocultivated with increasing numbers of NAF (A) or CAFs (B) for 4 d (gray columns) or 8 d (black columns). NAFs inhibited the growth of epithelial cells, statistically significant after 8 d of cocultivation; *, P < 0.05 (A, left graph). CAFs promoted the growth of epithelial cells statistically significant after 8 d of cocultivation (B, left graph). The growth of NAFs or CAFs in cocultivation was monitored by the measurement of GFP fluorescence. Increasing amounts of NAFs or CAFs showed a linear increase in GFP fluorescence (A, B, right graph). Error bars, SD of the means of three independent experiments. 1 × 103 PC-3 cells were cocultivated with increasing numbers of NAFs (C) or CAFs (D) for 4 d (gray columns) or 8 d (black columns). NAFs promoted the growth of PC-3 cells statistically significant after 4 d (*, P < 0.05) and after 8 d (**, P < 0.005; C, right graph), whereas CAFs had no influence on the growth of PC-3 cells (D, left graph). The growth of NAFs or CAFs in coculture was monitored by measuring of GFP fluorescence. Increasing amounts of NAFs showed a linear increase in GFP fluorescence (C, D, right graph). One representative experiment of three independent experiments. Error bars, SD of the means of duplicates. Nicole Paland et al. Mol Cancer Res 2009;7:1212-1223 ©2009 by American Association for Cancer Research