Hideki Itano, MDa, Bassem N. Mora, MDa, Wanjiang Zhang, MDa, Jon H

Slides:

Advertisements

Similar presentations

Effect of ventilator-induced lung injury on the development of reperfusion injury in a rat lung transplant model Marc de Perrot, MDa, Yumiko Imai, MDb,

Advertisements

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of metastatic lung tumors in a rat model Paul M. Petre, MDa, Frank A. Baciewicz, MDb, Stefan.

Prevention of lung ischemia–reperfusion injury by short hairpin RNA–mediated caspase- 3 gene silencing Yong-Xing Zhang, MD, Hong Fan, PhD, Yu Shi, MD,

Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

Zhao Jing, M. D. , Zhang Qiong, M. D. , Wang Yonggang, M. D

Ugonna N. Ihekweazu, BS, William D. Travis, MD, Raja M. Flores, MD

Time course and cellular localization of inducible nitric oxide synthases expression during cardiac allograft rejection Neil K Worrall, MD, Thomas P.

Liposome-mediated gene transfer to lung isografts

Effects of azithromycin and tanomastat on experimental bronchiolitis obliterans Katharina Krenn, MD, Matthias Gmeiner, MD, PhD, Patrick Paulus, MD, Nezir.

Uz Stammberger, MDa, Jürg Hamacher, MDb, Sven Hillinger, MDc, Ralph A

Inhibitory Effects of Calcitonin Gene-Related Peptides on Experimental Vein Graft Disease Xiaoning Zhang, MD, Jian Zhuang, MD, Hongsui Wu, MB, Zhihong.

Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T

Gene transfer of heat shock protein 70 protects lung grafts from ischemia-reperfusion injury Masafumi Hiratsuka, MD, Bassem N Mora, MD, Motoki Yano,

Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3 Se Jin Park,

Carbon monoxide reduces pulmonary ischemia–reperfusion injury in miniature swine Hisashi Sahara, MD, Akira Shimizu, MD, PhD, Kentaro Setoyama, DVM, PhD,

Volume 82, Issue 8, Pages (October 2012)

Baiya Krishnadasan, MD, Babu V

Controlled release of ascorbic acid from gelatin hydrogel attenuates abdominal aortic aneurysm formation in rat experimental abdominal aortic aneurysm.

Tumor necrosis factor-α production in whole blood after cardiopulmonary bypass: Downregulation caused by circulating cytokine-inhibitory activities J.

J. Hunter Mehaffey, MD, Eric J. Charles, MD, Ashish K

Hyperbaric oxygen as a chemotherapy adjuvant in the treatment of metastatic lung tumors in a rat model Paul M. Petre, MDa, Frank A. Baciewicz, MDb, Stefan.

Inhibition of complement C5 reduces local and remote organ injury after intestinal ischemia/reperfusion in the rat Koichiro Wada, Michael C. Montalto,

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Highly efficient ex vivo gene transfer to the transplanted heart by means of hypothermic perfusion with a low dose of adenoviral vector Carlo Pellegrini,

Chronic pulmonary artery occlusion increases alveolar fluid clearance in rats Zheng Wang, MD, Jin Xu, MD, Gang Ma, MD, Motoyasu Sagawa, MD, PhD, Miyako.

Specific inhibition of p38 mitogen-activated protein kinase with FR attenuates vascular proliferation in monocrotaline-induced pulmonary hypertension.

Role of TWIK-related potassium channel-1 in chronic rhinosinusitis

Chengpei Xu, MD, PhDa, Sheila Lee, BSa, Tej M

Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,

Nebulized nitrite protects rat lung grafts from ischemia reperfusion injury Toshihiro Okamoto, MD, PhD, Xiaoying Tang, MS, Allison Janocha, BS, Caral.

Dexamethasone Pretreatment Provides Antiinflammatory and Myocardial Protection in Neonatal Arterial Switch Operation Ruth Heying, MD, PhD, Edith Wehage,

Recombinant human hepatocyte growth factor transfection alleviates hyperkinetic pulmonary artery hypertension in rabbit models Wei Wang, MD, Kai Liu,

Activated protein C attenuates cardiopulmonary bypass–induced acute lung injury through the regulation of neutrophil activation Sachiko Yamazaki, MD,

Carbon monoxide–saturated preservation solution protects lung grafts from ischemia– reperfusion injury Junichi Kohmoto, MD, Atsunori Nakao, MD, Ryujiro.

Oral hydrogen water prevents chronic allograft nephropathy in rats

Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome Yongguang Lu, Lang Li, Hua Yan, Qiang.

Effect of ventilator-induced lung injury on the development of reperfusion injury in a rat lung transplant model Marc de Perrot, MDa, Yumiko Imai, MDb,

Improvement of rejection-induced diastolic abnormalities in rat cardiac allografts with inducible nitric oxide synthase inhibition Pablo F. Soto, MDa,

Denis W. Harkin, MD, Aires A. B

Christina I. Selinger, PhD, Wendy A

Moderate hypothermia during cardiopulmonary bypass increases intramyocardial synthesis of heat shock protein 72 Ma Qing, MD, Jaime F. Vazquez-Jimenez,

Decreased lung ischemia-reperfusion injury in rats after preoperative administration of cyclosporine and tacrolimus B. Krishnadasan, MD, B. Naidu, MBBS,

Volume 86, Issue 4, Pages (October 2014)

Recipient intramuscular gene transfer of active transforming growth factor-β1 attenuates acute lung rejection Takashi Suda, MD, Franco D’Ovidio, MD,

Tsutomu Tagawa, MDa, Takashi Suda, MDa, Niccolò Daddi, MDa, Benjamin D

Expression of vascular endothelial growth factor and its receptors is increased, but microvascular relaxation is impaired in patients after acute myocardial.

Increased cerebral and renal endothelial nitric oxide synthase gene expression after cardiopulmonary bypass in the rat C. David Mazer, MD, Francoise.

Adenovirus encoding soluble tumor necrosis factor α receptor immunoglobulin prolongs gene expression of a cotransfected reporter gene in rat lung Takashi.

Validating the use of short interfering RNA as a novel technique for cell-specific target gene knockdown in lung ischemia–reperfusion injury Heather.

Despite some expression of folate receptor α in human mesothelioma cells, internalization of methotrexate is predominantly carrier mediated Nushmia Z.

Samer A. Kanaan, MD, Benjamin D

CD4+ T lymphocytes mediate acute pulmonary ischemia–reperfusion injury

Evidence of human non-α-galactosyl antibodies involved in the hyperacute rejection of pig lungs and their removal by pig organ perfusion Paolo Macchiarini,

Increased nitric oxide levels in exhaled air of rat lung allografts

Ex vivo transfection of pulmonary artery segments in lung isografts

A.K.I. Kuroki, Masayuki Iyoda, Takanori Shibata, Tetsuzo Sugisaki

Partial liquid ventilation for acute allograft dysfunction after canine lung transplantation Hideki Itano, MD, Motoi Aoe, MD, Shingo Ichiba, MD, Motohiro.

In Vivo Tracking of Mesechymal Stem Cells Using Fluorescent Nanoparticles in an Osteochondral Repair Model Jong Min Lee, Byung-Soo Kim, Haeshin Lee,

Nicorandil, a potent adenosine triphosphate–sensitive potassium-channel opener, ameliorates lung allograft reperfusion injury Motohiro Yamashita, MD,

Lamotrigine improves cerebral outcome after hypothermic circulatory arrest: A study in a chronic porcine model Vesa Anttila, MDa, Jussi Rimpiläinen,

Exacerbation of systemic inflammation and increased cerebral infarct volume with cardiopulmonary bypass after focal cerebral ischemia in the rat H. Mayumi.

Endobronchial transfection of naked TGF-β1 cDNA attenuates acute lung rejection Franco D’Ovidio, MD, Motoki Yano, MD, Jon H Ritter, MD, Thalachallour.

Overexpression of endothelial nitric oxide synthase increases skeletal muscle blood flow and oxygenation in severe rat hind limb ischemia Lucy S Brevetti,

β-chemokine function in experimental lung ischemia-reperfusion injury

Motoki Yano, MDa, Carlos H. R. Boasquevisque, MDa, Ronald K

Postbypass effects of delayed rewarming on cerebral blood flow velocities in infants after total circulatory arrest Rosendo A. Rodriguez, MD, PhDa (by.

Discussion The Journal of Thoracic and Cardiovascular Surgery

Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery Robert A. Larson, MD, Mina.

Similar neurobehavioral outcome after valve or coronary artery operations despite differing carotid embolic counts Michael J. Neville, MDa, John Butterworth,

Endogenous interleukin-4 and interleukin-10 regulate experimental lung ischemia reperfusion injury Alexander S Farivar, MD, Baiya Krishnadasan, MD, Babu.

Presentation transcript:

Lipid-mediated ex vivo gene transfer of viral interleukin 10 in rat lung allotransplantation Hideki Itano, MDa, Bassem N. Mora, MDa, Wanjiang Zhang, MDa, Jon H. Ritter, MDb, Timothy J. McCarthy, PhDc, Nelson S. Yew, PhDd, T. Mohanakumar, PhDa, G.Alexander Patterson, MDa The Journal of Thoracic and Cardiovascular Surgery Volume 122, Issue 1, Pages 29-38 (July 2001) DOI: 10.1067/mtc.2001.114636 Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Semiquantitative RT-PCR analysis of vIL-10 mRNA transgene expression in rat lung allografts. The peak areas of bands were expressed as a relative ratio of vIL-10/glyceraldehyde-3-phosphate dehydrogenase (GA3PDH). The gel image shows vIL-10 bands from groups 1 and 2 but not from group 3. The relative ratio of vIL-10/glyceraldehyde-3-phosphate dehydrogenase in group 1 was significantly higher than that in group 2 (P =.0050 vs group 2). Values are means ± SD of 6 or 7 animals per group. PC, Positive control. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Immunohistochemistry for transgene vIL-10. Transgene vIL-10 protein was detected by means of immuno-histochemistry in bronchial endothelial cells, alveolar epithelial cells, and alveolar macrophages. (Original magnifications: A, 200×; B, 200×; C, 400×; D, 400×.) The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Histologic rejection score. The sections were graded separately for both acute vascular rejection (A-score) and airway rejection (B-score). The acute vascular rejection score in group 1 was significantly lower than that in group 3 (P =.042 vs group 3, Kruskal-Wallis test). There were no significant differences (NS) in airway rejection scores. Bars show median values. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Arterial blood oxygen tension (PaO2). PaO2 obtained 5 minutes after right hilar crossclamping is shown. Each PaO2 in groups 1 (114.06 ± 61.05 mm Hg) and 2 (108.58 ± 35.7 mm Hg) was significantly higher than that in group 3 (66.4 ± 8.22 mm Hg; P =.020 and P =.023 vs group 3). Values are means ± SD of 6 or 7 animals per group. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Exhaled NO levels from rat lung allografts. Exhaled NO levels in group 2 (5.150 ± 6.38 ppb) were significantly lower than those in group 3 (13.517 ± 10.4 ppb; P =.020 vs group 3). Exhaled NO levels in normal lungs (NL ; 0.00 ± 0.0 ppb) were significantly lower than those in groups 1 and 3 (*P <.005, respectively). Values are means ± SD of 5 to 7 animals per group. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 6 Semiquantitative RT-PCR analysis of IL-2 mRNA expression in rat lung allografts. The peak areas of the bands were expressed as the relative ratio of IL-2/glyceraldehyde-3-phosphate dehydrogenase (GA3PDH). IL-2 mRNA expression in group 1 and in normal lungs was significantly lower than that in group 3 (P =.039 and P =.0014 vs group 3). IL-2 mRNA expression in group 2 was lower than that in group 3, trending toward significance (P =.068 vs group 3). Values are means ± SD of 6 or 7 animals per group. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

Fig. 7 RT-PCR analysis for mRNA expression of tumor necrosis factor (TNF) α, iNOS, IFN-γ, and endogenous IL-10 in rat lung allografts. Normal lung (NL) mRNA expression in these cytokines was significantly lower than that in other groups (*P <.05, respectively). There were no significant differences between groups 1, 2, and 3. Values are means ± SD of 4 to 7 animals per group. GA3PDH, Glyceraldehyde-3-phosphate dehydrogenase. The Journal of Thoracic and Cardiovascular Surgery 2001 122, 29-38DOI: (10.1067/mtc.2001.114636) Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions