Dual apoptotic signaling pathways triggered by IMMU-140: Anti–HLA-DR- and SN-38–mediated signals. Dual apoptotic signaling pathways triggered by IMMU-140:

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Dual apoptotic signaling pathways triggered by IMMU-140: Anti–HLA-DR- and SN-38–mediated signals. Dual apoptotic signaling pathways triggered by IMMU-140: Anti–HLA-DR- and SN-38–mediated signals. Cells were plated and IMMU-114 and IMMU-140 were added as described in Materials and Methods and Supplementary Data. Both IMMU-114 and IMMU-140 were added at equal 10 nmol/L protein doses for the indicated times before cells were harvested for Western blot analysis. All incubation times shown in blue indicate IMMU-114 and those in red indicate IMMU-140. A, JVM-3 (CLL), MN-60 (ALL), GDM-1 (AML), MOLM-14 (AML), and A-375 (melanoma) cell lines analyzed for full-length PARP (PARP FL), cleaved PARP fragment (Cleaved), and phosphorylated ERK1/2 (p-ERK1/2) levels. Total ERK1/2 and β-actin served as protein loading controls. B, Levels of dsDNA breaks were determined by release of phosphorylated histone H2A.X (p-H2A.X). Assessment of changes in dsDNA breaks was calculated as ratios relative to untreated control, normalized to β-actin protein loading control (Δp-H2A.X). Thomas M. Cardillo et al. Mol Cancer Ther 2018;17:150-160 ©2018 by American Association for Cancer Research