INDUCED PLURIPOTENT STEM CELLS (IPSCs) IPSCs generation does not involve technically more tedious methodologies There are no bioethical issues surrounding the IPSCs as a continuous source of autologous & disease specific pluripotent SCs. IPSCs reprogrammed from somatic cells via transgenic expression of pluripotent determining Transcription Factors possess similar differentiation capacity Reprogramming process: Elite Model and Stochastic Model

REPROGRAMMING PROCESS Elite Model: not all somatic cells were re-programmable - e.g.: presence of Multi-lineage-differentiating Stress Enduring (MUSE) cells is predominantly responsible for IPSCs generation from human fibroblast. Stochastic model predicted that all somatic cell types might be reprogrammed with the involvement of epigenetic alterations enrouted to pluripotency. - e.g.: reprogramming of skin fibroblast, dental pulp, peripheral blood cells, T-cells, BM-cells, skeletal myoblast.