EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3 cells. EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3.

Slides:

Advertisements

Similar presentations

KG-501 suppresses NSCLC/IL-1β CM–induced migration of HUVECs by regulating IL-1β–induced CXC chemokine gene expression in NSCLC cells. KG-501 suppresses.

Advertisements

DNA damage induces complex signal activation dynamics across many signaling pathways. DNA damage induces complex signal activation dynamics across many.

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived PC12 cells. DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

p38 MAPK activation is required for phosphorylation of Akt at Ser473.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Thiocolchicoside inhibits TNF-dependent IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. Thiocolchicoside inhibits.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.

Accumulation of ubiquitinated MDM2 following PN treatment requires the PIKK ATM. A, Western blots of nuclear extract proteins from ZR-75-1 cells treated.

The sesquiterpene lactone PN up-regulates MDM2 and p53 proteins.

Quantification of NMT knockdown.

FOXO3a phosphorylation and expression.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

Identification of NSC as a FADD-kinase inhibitor.

AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2. AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2.

Dephosphorylation of pocket proteins in response to AG1024.

DNA damage signaling regulates mutant p53 ubiquitination.

Imatinib mesylate inhibits PDGF-mediated ERK and Akt activation.

4-OHE2 induces neoplastic cell transformation and PCNA expression.

NF-kB binds to the NF-κB binding site immediately adjacent to the AT-rich regulatory region. NF-kB binds to the NF-κB binding site immediately adjacent.

PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.

Inhibitory effects of nobiletin and resveratrol on LPS/IFN-γ-induced NO generation in RAW cells. Inhibitory effects of nobiletin and resveratrol.

Effects of recombinant human HPSE on Topo I activity in 231BR3 nucleoli. Effects of recombinant human HPSE on Topo I activity in 231BR3 nucleoli. Representative.

Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.

Lamellarin D induces cell death through a Fas-independent pathway.

Clathrin-dependent, constitutive endocytosis of DR4 and DR5 in TRAIL-resistant cells. Clathrin-dependent, constitutive endocytosis of DR4 and DR5 in TRAIL-resistant.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Induction of cytotoxic activity in humanized SCC3 tumor-bearing mice treated with anti-PD-1 antibody. Induction of cytotoxic activity in humanized SCC3.

A. A. Honokiol inhibits TNF-induced NF-κB activation, IκBα phosphorylation, and IκBα degradation. Honokiol inhibits TNF-induced activation of NF-κB. H1299.

MDA-468TR-PTEN (with and without 100 ng/ml doxycycline) and MDA-468TR-vector (with dox) were serum starved overnight and the following day treated for.

Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells. Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells.

N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.

Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

Synergistic cytotoxicity of 17AAG and TNF treatment in human lung cancer cell lines. Synergistic cytotoxicity of 17AAG and TNF treatment in human lung.

Butyrate inhibits IFN-γ-induced STAT1 DNA binding.

Staurosporine induces changes in MMP

Ketogenic diets combined with fractionated radiation treatment results in decreased immunoreactive PCNA in tumor tissue. Ketogenic diets combined with.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Interaction of Cdx1 and Cdx2 with the putative Cdx-binding sites of the human DSC2 promoter. Interaction of Cdx1 and Cdx2 with the putative Cdx-binding.

Frequency of misrepair studies with increasing concentrations of antibodies against NHEJ repair proteins. Frequency of misrepair studies with increasing.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

Ligation efficiency is abrogated with increasing concentration of antibodies against NHEJ proteins. Ligation efficiency is abrogated with increasing concentration.

Changes in signal transduction pathway induced by gefitinib.

Expression of the cell death markers activated caspase-3 and LC3 I/II in TUBO cells. Expression of the cell death markers activated caspase-3 and LC3 I/II.

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

The Ser124 site of Cdc25A is required for Cdc25A degradation in response to I3C induction. The Ser124 site of Cdc25A is required for Cdc25A degradation.

Osteoactivin expression is required for the invasive phenotype of in vivo selected bone metastatic 4T1 breast cancer cells. Osteoactivin expression is.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

CHL-induced apoptosis in HCT116 cells involves the activation of procaspase-8 and procaspase-6, with cleavage of nuclear lamins. CHL-induced apoptosis.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

Effect of bevacizumab on the proliferation of A2780 cells.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Expression and induction of HER2 and HPSE in 231BMBC cells.

BEZ235 induces accelerated senescence after radiation (IR) in vitro.

Effect of MIF siRNA on the production of MMP-13 induced by LPA

Posttranslational phosphorylation of p53 by platinum drugs in ovarian tumor cells. Posttranslational phosphorylation of p53 by platinum drugs in ovarian.

Detection of protein levels of Cdc25AWT and its mutation derivatives in breast cancer cells with I3C treatment. Detection of protein levels of Cdc25AWT.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

EGF induces HPSE nucleolar localization in human BMBC cells.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

T3 or T4 induces MAPK activation in myeloma cells.

Effects of NMT siRNAs on MARCKS and c-Src.

I3C reduces the level of Cdc25A protein in breast cancer cells.

WEE1 inhibition followed by TRAIL treatment results in apoptotic cell death in MB231 cells. WEE1 inhibition followed by TRAIL treatment results in apoptotic.

Sp1 complementation assay.

Presentation transcript:

EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3 cells. EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3 cells. A, cell fractionation was done as described in Materials and Methods. The fractions were subjected to Western blotting applying anti-HPSE antibody (1453). Purity of fractions was verified using nucleoli-specific (anti-fibrillarin), nuclear-specific (anti-SC35), and cell membrane–specific (anti–integrin β1) antibodies. S, serum-free only (control); E, EGF treatment. B, a representative agarose gel showing EGF-induced Topo I activity. Nucleolar Topo I converts supercoiled DNA (Sc, provided by Topo I assay kit, TopoGen, Inc.) into a relaxed DNA form. Samples were treated with EGF (100 ng/mL) as indicated. Nucleoli were derived from (A) above. C, Topo I activity assays. Briefly, nucleoli were isolated from 231P and 231BR3 cells following treatment with or without EGF (100 ng/mL) for 30 min as per A. Topo I activity was determined by its ability to produce the relaxed DNA form, and was calculated as a ratio of relaxed/supercoiled DNA normalized against nontreated 231P. Three independent experiments were done. **, P < 0.001. Lixin Zhang et al. Mol Cancer Res 2010;8:278-290 ©2010 by American Association for Cancer Research