Immunohistochemical staining of primary and metastatic prostatic carcinomas with anticyclin D1 and anti-Ki67 monoclonal antibodies. Immunohistochemical.

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Immunohistochemical staining of primary and metastatic prostatic carcinomas with anticyclin D1 and anti-Ki67 monoclonal antibodies. Immunohistochemical staining of primary and metastatic prostatic carcinomas with anticyclin D1 and anti-Ki67 monoclonal antibodies. A, primary tumor displaying a strong nuclear staining in tumor cells. Note the lack of immunoreactivities observed in the stroma elements. B, high-powered microscopic view of the same case illustrated in A. Note the intense staining of the positive tumor nuclei. C, primary tumor with undetectable levels of cyclin D1, representative of the negative phenotype cases. D, metastatic prostate cancer to bone displaying a positive immunophenotype in the nulcei of the majority of invasive tumor cells. E, high-powered microscopic view of the same case illustrated in D. F, representative case of a high Ki67 proliferative index in a prostate cancer. Note the intense immunoreactivities in invasive tumor cells. Original magnifications: A and D, ×200; B, C, E, and F, ×400. Marija Drobnjak et al. Clin Cancer Res 2000;6:1891-1895 ©2000 by American Association for Cancer Research