Immunoblot assay (left panel) and whole-cell radioimmunoprecipitation (RIP) assay (right panel) with preexacerbation serum (lanes A) and postexacerbation.

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New Concepts in Microbiology of Exacerbations of COPD Sanjay Sethi MD Professor Pulmonary, Critical Care and Sleep Medicine University at Buffalo, SUNY.

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Presentation transcript:

Immunoblot assay (left panel) and whole-cell radioimmunoprecipitation (RIP) assay (right panel) with preexacerbation serum (lanes A) and postexacerbation serum (lanes B) from an adult with COPD who experienced an exacerbation due to nontypeable H. influenzae. Immunoblot assay (left panel) and whole-cell radioimmunoprecipitation (RIP) assay (right panel) with preexacerbation serum (lanes A) and postexacerbation serum (lanes B) from an adult with COPD who experienced an exacerbation due to nontypeable H. influenzae. Assays were performed with the homologous infecting strain. The positions of molecular mass standards are noted (in kilodaltons) to the left of each panel. Note that immunoblot assays detect antibodies to many bands with minimal difference between pre- and postexacerbation sera. By contrast, whole-cell radioimmunoprecipitation assays with the same sera show the development of new antibodies to P2 (arrow) and higher-molecular-mass proteins following the exacerbation. Sanjay Sethi, and Timothy F. Murphy Clin. Microbiol. Rev. 2001; doi:10.1128/CMR.14.2.336-363.2001