Generation and characterization of LSCCs generated by Trp53−/− and Keap1−/−;Trp53−/− tracheal epithelial cells. Generation and characterization of LSCCs.

Slides:

Advertisements

Similar presentations

PIK3CA gene amplification in glioblastoma and squamous cell carcinoma (SCC) of the lung. PIK3CA gene amplification in glioblastoma and squamous cell carcinoma.

Advertisements

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

SCC244 significantly inhibited c-Met–driven tumor growth in cancer CDX models. SCC244 significantly inhibited c-Met–driven tumor growth in cancer CDX models.

T3 increased J7-TRα1 cell migration and proliferation both in vitro and in vivo. T3 increased J7-TRα1 cell migration and proliferation both in vitro and.

Development of preclinical models for the guidance of precision medicine. Development of preclinical models for the guidance of precision medicine. A,

GM-CSF induces tumor cell EMT and stemness.

Adi F. Gazdar, MD, Fred R. Hirsch, MD, PhD, John D. Minna, MD

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

SOX2 Is a Marker for Stem-like Tumor Cells in Bladder Cancer

Microarray analysis of phospho-Twist1–responsive genes.

SOX2 Is a Marker for Stem-like Tumor Cells in Bladder Cancer

Volume 13, Issue 10, Pages (December 2015)

Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Increase in beige/brown adipocyte characteristics.

Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model. Bioluminescence imaging facilitates detection.

Down-regulation of LATS1 promotes the formation of tumors enriched in basal-like features. Down-regulation of LATS1 promotes the formation of tumors enriched.

Loss of Keap1 contributes to LSCC pathogenesis by activating the NRF2–ROS pathway. Loss of Keap1 contributes to LSCC pathogenesis by activating the NRF2–ROS.

Effect of antiangiogenic TKIs and rMVA–CEA–TRICOM vaccine on tumor compactness, tight junctions, and intratumoral pressure in the MC38-CEA model. Effect.

The Mucinous Lung Tumor Signature is highly enriched in colorectal, stomach, pancreatic, lung (adenocarcinoma; LUAD), and breast cancers The Mucinous Lung.

(A) Schematic representation of the conditional Lats2 locus.

Expression of p110γ isoform in macrophages promotes tumor growth and angiogenesis. Expression of p110γ isoform in macrophages promotes tumor growth and.

Genetic EGFR ablation in K-RAS–mutated lung AC reduces tumor growth

TH-302 has antileukemia activity in an in vivo primary AML xenograft murine model. TH-302 has antileukemia activity in an in vivo primary AML xenograft.

IL32 is highly expressed in mycosis fungoides lesional skin.

SDF-1/CXCR4 axis is involved in CD133+ tumor cell metastasis toward a lymphatic metastasis niche. SDF-1/CXCR4 axis is involved in CD133+ tumor cell metastasis.

IL6 mRNA is not detected in metastatic prostate cancer cells.

EHop-016 and INK128 treatment of myxofibrosarcoma xenografts in NSG mice. EHop-016 and INK128 treatment of myxofibrosarcoma xenografts in NSG mice. A,

Quercetin induces arrest in G1 phase of cell cycle in P39 xenografts.

Quercetin induces apoptosis in P39 xenografts.

CHCHD2 and EGFR protein expression in NSCLC

Depletion of UCP1 and Myf5 population reduced xenograft growth.

Cisplatin plus PTUPB decreases proliferation and angiogenesis but increases apoptosis as determined by immunohistochemical (IHC) analysis. Cisplatin plus.

MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting Sox2. MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

PD-L1 expressed on edited T3 sarcoma cells prevents their immune elimination. PD-L1 expressed on edited T3 sarcoma cells prevents their immune elimination.

IDO deficiency delays the development of pulmonary metastases.

In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. A,

MEDI4736 shows antitumor activity in xenograft mouse models of human cancer. MEDI4736 shows antitumor activity in xenograft mouse models of human cancer.

GM-CSF is required for CA-MSC–induced tumor metastasis.

Effect of the crystal form and solid dispersion preparations of KRN633 on the growth of human tumor xenografts in mice. Effect of the crystal form and.

Example inverse FF-OCT images (left column) and corresponding histology images (right column) of ovarian metastases. Example inverse FF-OCT images (left.

Tumor cell clusters arise from cellular aggregation.

Representative FFPE sections of subcutaneous xenografts derived from HBEC3p53,KRAS and HBEC3p53,KRAS,MYC. Representative FFPE sections of subcutaneous.

Reduced klotho expression in pancreatic cancer.

Expression of versican promoted breast cancer cell tumor formation and self-renewal in vivo. Expression of versican promoted breast cancer cell tumor formation.

CD44 depletion blocks tumor cell aggregation and lung metastasis in vivo. CD44 depletion blocks tumor cell aggregation and lung metastasis in vivo. A,

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Expression of HO-1 by the FAP+/F4/80+ tumoral macrophages.

Fig. 1 Number of somatic mutations in representative human cancers, detected by genome-wide sequencing studies. Number of somatic mutations in representative.

The CCR5+ population of SUM-159 cells is enriched with tumor-initiating cells. The CCR5+ population of SUM-159 cells is enriched with tumor-initiating.

SPARC is required for spontaneous metastasis

Gastric, lung, and HPV-related cancer incidence and model fits.

Reduced expression of the tumor suppressor PTEN occurs in tumors with both squamous cell and adenocarcinoma histology. Reduced expression of the tumor.

Validation of NAA's cancer specificity in lung cancer.

LSECtin, expressed by B16 cells, inhibits the tumor-specific immune responses both in vivo and in vitro. LSECtin, expressed by B16 cells, inhibits the.

Relative frequencies of FGFR aberrations in non–small cell lung carcinoma. Relative frequencies of FGFR aberrations in non–small cell lung carcinoma. A,

Cabozantinib causes significant tumor cell elimination in vivo, but modest apoptosis induction in vitro. Cabozantinib causes significant tumor cell elimination.

In vivo shRNA screening in PDXs from 3 patients with melanoma.

GCS-100 selectively kills KRAS-addicted lung tumors.

VEGF-A inhibition impedes proliferation in Amppos tumors.

Identification of a subpopulation of highly metastatic pancreatic cancer cells. Identification of a subpopulation of highly metastatic pancreatic cancer.

Validation of KMT2D function in MM23, MM2, and MM25 PDXs

AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. A,

mRNA expression of PARP1 in SCLC cell lines and solid tumors.

MSCs elicit EMT, invasion of carcinoma cells, and increased tumor initiation of xenografts. MSCs elicit EMT, invasion of carcinoma cells, and increased.

EC-derived SP cells are targeted by CD30.CAR T cells.

SCLC initiated from pulmonary neuroendocrine cells metastasizes without upregulating NFIB. A and B, Mouse models of SCLC. Rb1flox/flox;Trp53flox/flox;p130flox/flox;R26mTmG.

Tumor progression is required prior to metastasis of SCLC in both CMV TKO and CGRP TKO mice. Tumor progression is required prior to metastasis of SCLC.

PD-L1 expression is not associated with PTEN expression status in melanomas. PD-L1 expression is not associated with PTEN expression status in melanomas.

SCLC in CMV TKO mice arises from an alternative cell of origin.

Presentation transcript:

Generation and characterization of LSCCs generated by Trp53−/− and Keap1−/−;Trp53−/− tracheal epithelial cells. Generation and characterization of LSCCs generated by Trp53−/− and Keap1−/−;Trp53−/− tracheal epithelial cells. A, Tumor-generating strategy (adenocarcinoma). Trp53f/f;R26tdTomato mice were intranasally instilled with Ad-Cre and observed for tumor formation. B, Representative H&E and IHC sections from tumors arising from Ad-Cre–instilled Trp53−/− mice in A. Expression of TTF1 and KRT5 is shown. P-LUAD, Trp53-/- lung adenocarcinoma. C, Tumor-generating strategy (adenocarcinoma). tdTomato+ lung cells from Ad-Cre–instilled Trp53f/f;R26tdTomato mice were FACS sorted and transplanted subcutaneously into NOD/SCID IL2Rgammanull (NSG) mice for tumor formation. D, Representative H&E-stained sections from tumors arising in NSG mice transplanted with Trp53−/− lung cells in (C). E, Tumor-generating strategy (squamous cell carcinoma). Bulk tracheal epithelial cells (10,000–30,000) were transduced overnight with Ad-Cre viruses in vitro and transplanted into NSG mice. F, Incidence of tumor formation by WT, Keap1−/−, Trp53−/−, and Keap1−/−;Trp53−/− tracheal epithelial cells. G, Representative H&E and IHC sections from tumors arising from a human LSCC patient–derived xenograft and K/P- and P-LSCC tumors. Expression of KRT5, SOX2, and TTF1 is shown. H, Relative mRNA expression of Krt5, p63, Sox2, Sftpc, and Cgrp in cells sorted from Keap1−/−;Trp53−/− LSCC (K/P-LSCC), Trp53−/− LSCC (P-LSCC), KrasG12D;Trp53−/− LUAD (43), and Rb−/−;Trp53−/−;p130−/− small cell lung cancer (SCLC; ref. 75; N = 3). I, Representative gross tumor and stained sections of lung metastases from subcutaneously transplanted K/P-LSCC cells (all scale bars, 100 μm). J, Incidence of lung cancer subtypes by different tumor-generating strategies. Youngtae Jeong et al. Cancer Discov 2017;7:86-101 ©2017 by American Association for Cancer Research