CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial.

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CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. A, confocal microscopy images of HUVEC or 786-0 cells embedded in fibrin clots for 1 or 2 hours after immunostaining with anti-CLIC1 (red). Nuclei are stained with draq5 (blue). B, confocal microscopy images of 24-hour fibrin-embedded HUVEC and 786-0 cells after immunostaining for β3 integrin (green) and CLIC1 (red). Nuclei are stained with draq5 (blue). C, fibrin-embedded 786-0 cells transformed with scrambled (shSCR, top) or β3 shRNA (shβ3, bottom) were fixed after indicated times and probed for CLIC1 (green) and F-actin (red). Nuclei are stained with draq5 (blue). Scale bars, 10 μm. Lisa A. Gurski et al. Mol Cancer Res 2015;13:273-280 ©2015 by American Association for Cancer Research