Lung tumors in KrasLA1 mice are fibrotic.

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Lung tumors in KrasLA1 mice are fibrotic. Lung tumors in KrasLA1 mice are fibrotic. A, tissue scaffold created by detergent-based decellularization of lungs from a KrasLA1 mouse with areas of hyperdense stroma in tumoral regions that contain dense collagen bundles by TEM analysis (inset). Bar size, 1 μm. B, Masson trichrome staining (left) of collagen (blue) in lung tumor from a KrasLA1 mouse. T, tumor. N, normal lung. Bar size, 200 μm. C, TEM analysis (right) of lung tumor from a KrasLA1 mouse. T, tumor cell. Arrowhead, fibroblastic cell. Arrow, collagen bundles. D, immunocytochemical analysis of type I collagen (red) in CAFs grown in monolayer culture on plastic. Bar size, 100 μm. E, SHG images of 344SQ cells seeded alone or with CAFs into collagen gels. Collagen (green). Bar size, 200 μm. F, Young modulus for cells entrapped in collagen gels. Gels containing CAFs (n = 4) had significantly higher Young modulus as compared with cell-free collagen gels (n = 5), P < 0.0001. Daniela Pankova et al. Mol Cancer Res 2016;14:287-295 ©2016 by American Association for Cancer Research