Validation of GHSR, SST, and ZIC1 in hrHPV-positive cervical scrapes and their relation to duration of lesion existence and DNA copy number aberrations.

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Validation of GHSR, SST, and ZIC1 in hrHPV-positive cervical scrapes and their relation to duration of lesion existence and DNA copy number aberrations. Validation of GHSR, SST, and ZIC1 in hrHPV-positive cervical scrapes and their relation to duration of lesion existence and DNA copy number aberrations. ROC curves of GHSR, SST, and ZIC1 for CIN3+ detection in hrHPV-positive cervical scrapes (A). Methylation levels of GHSR (B), SST (C), and ZIC1 (D) in hrHPV-positive cervical scrapes from women with CIN2/3 with a known duration of previous hrHPV infection (PHI), as a proxy for the duration of lesion existence; Early CIN2/3 corresponds to <5-year PHI; Advanced CIN2/3 corresponds to ≥5-year PHI. Methylation levels of GHSR (E), SST (F), and ZIC1 (G) in hrHPV-positive cervical scrapes in relation to the presence of a chromosomal gain of respective chromosomal region (i.e., chr. 3q26, chr. 3q28, and chr. 3q24, respectively) in the underlying advanced CIN2/3 lesions. The x-axis displays the different groups [i.e., with (+3q gain) or without (−3q gain) 3q gain] and the y-axis displays the log2-transformed Cq ratios representing methylation levels; *, P < 0.05; ***, P < 0.0005. Wina Verlaat et al. Clin Cancer Res 2017;23:3813-3822 ©2017 by American Association for Cancer Research