Reproducibility of CD4+ and CD8+ T-cell enumeration with cryopreserved PBMC after variable periods of storage in liquid nitrogen. Reproducibility of CD4+ and CD8+ T-cell enumeration with cryopreserved PBMC after variable periods of storage in liquid nitrogen. Multiple aliquots from a single donor were tested after 1 to 15 months of storage in liquid nitrogen. The graphs show the results of the laboratory with typical performance. The lines represent the regression analysis of results over time. Adriana Weinberg et al. Clin. Vaccine Immunol. 2009; doi:10.1128/CVI.00342-08