Volume 9, Issue 10, Pages 1436-1439 (October 2016) Significant Improvement of Cotton Verticillium Wilt Resistance by Manipulating the Expression of Gastrodia Antifungal Proteins  Yiqin Wang, Chengzhen Liang, Shenjie Wu, Xueyan Zhang, Jiuyou Tang, Guiliang Jian, Gaili Jiao, Fuguang Li, Chengcai Chu  Molecular Plant  Volume 9, Issue 10, Pages 1436-1439 (October 2016) DOI: 10.1016/j.molp.2016.06.013 Copyright © 2016 The Authors Terms and Conditions

Figure 1 GAFPs Family Analysis and Transgenic Cotton Expressing SG4 Shows Enhanced Verticillium Wilt Resistance. (A) Evolutionary relationship of GAFP1–4, which can be divided into two subgroups. (B and C) In vitro antifungal activity assay of G1–4 against Trichoderma viride (B) and Verticillium dahliae (C). PC, positive control; NC, negative control; scale bar, 2 cm. (D) Both the signal peptides of GAFP1 and GAFP4 could target the fusion protein to the apoplastic regions. After treatment with 0.3 g ml−1 sucrose solution to induce plasmolysis, for constructs without the signal peptides, most of the fusion protein was stranded internal to the cell membrane. Scale bar, 10 μm. (E and F) SG4-expressing transgenic cotton lines in R15 and Zhong24 background in the Verticillium wilt disease nurseries in Yuncheng (E) and Anyang (F), respectively. Scale bars, 20 cm (E) and 25 cm (F). (G) SG4-expressing transgenic cotton lines in Zhong24 background in the Verticillium wilt disease nursery in Anyang, 2013. (H) Disease resistance evaluation of transgenic cotton expressing SG1 and SG4. The evaluation was carried out during the growing seasons of 2013–2015 in the Verticillium wilt disease nurseries in three different geographical locations of China (Yuncheng, Langfang, and Anyang). SC, susceptible control; IR, infection rate (%); RDI, relative disease index; RT, resistance type; HR, high resistance level; R, resistance level; T, tolerance level; S, susceptibility level. One-way ANOVA followed by Duncan's multiple range test was used to compare the significant levels between different lines, and different letters in the footnote “a, b, c” represent significant differences (P < 0.05). Molecular Plant 2016 9, 1436-1439DOI: (10.1016/j.molp.2016.06.013) Copyright © 2016 The Authors Terms and Conditions