FACS analyses of the inhibitory effect induced by mixed docetaxel, gefitinib, and cyclopamine on EGF plus SHHNp–stimulated PC3 cells. FACS analyses of the inhibitory effect induced by mixed docetaxel, gefitinib, and cyclopamine on EGF plus SHHNp–stimulated PC3 cells. The inhibitory effect induced by drugs on the progression of EGF plus SHHNp–stimulated PC3 cells in the cellular cycle was investigated by flow cytometric analysis. The stimulated PC3 cells were untreated or treated with the indicated concentrations of 1 or 2 nmol/L docetaxel, 0.1 μmol/L gefitinib, and 1 μmol/L cyclopamine, alone or in combination, for 2 d. At the end of incubation time, the cells were prepared as described in Materials and Methods and the cell cycle distributions were assessed by FACS analyses. Representative results obtained from three separate experiments. Murielle Mimeault et al. Mol Cancer Ther 2007;6:967-978 ©2007 by American Association for Cancer Research