UNC569 reduces colony formation in Jurkat and 697 cell lines.

Slides:

Advertisements

Similar presentations

DNA content flow cytometric histograms of propidium iodide–stained A549 cells. DNA content flow cytometric histograms of propidium iodide–stained A549.

Advertisements

Localization of H2B-GFP protein in HeLa cells.

by Susan E. Shetzline, Ravikumar Rallapalli, Kelley J

Deregulated expression of the Myc cellular oncogene drives development of mouse “Burkitt-like” lymphomas from naive B cells by Delin Zhu, Chen Feng Qi,

by Veerendra Munugalavadla, Jovencio Borneo, David A

Hyaluronate-Enhanced Hematopoiesis: Two Different Receptors Trigger the Release of Interleukin-1β and Interleukin-6 From Bone Marrow Macrophages by Sophia.

CD19-targeting liposomes containing imatinib efficiently kill Philadelphia chromosome–positive acute lymphoblastic leukemia cells by Masamitsu Harata,

Cellular activity and selectivity of ARS-853.

TGF-β combined with M-CSF and IL-4 induces generation of immune inhibitory cord blood dendritic cells capable of enhancing cytokine-induced ex vivo expansion.

Expression of FUT9 supports tumor development

Inhibition of FGFR signaling and tumor growth in SNU-16 xenograft model by administration of E7090. Inhibition of FGFR signaling and tumor growth in SNU-16.

The BET inhibitor JQ‐1 in combination with the MEK inhibitor PD synergistically impairs cell proliferation and induces apoptosis of NRAS‐mutant.

The fluorescent receptor chains exhibit their characteristic fluorescent signatures: images and spectra of human IFN-γR2/GFP and IFN-γR2/EBFP transfected.

The HDAC3 inhibitor suppresses PTEN‐deficient prostate cancer growth

Characterization of immunosorted NCAM1+ subpopulation A

Time-resolved colocalization studies of S100A4 and MYH9 in activated NK cells. Time-resolved colocalization studies of S100A4 and MYH9 in activated NK.

LIR‐mediated cross talk between cluster formation and the autophagy machinery LIR‐mediated cross talk between cluster formation and the autophagy machinery.

Inhibition of ROCK activity rescues formation of polarized cysts in the absence of DDR1 signaling. Inhibition of ROCK activity rescues formation of polarized.

Adipocytes sequester daunorubicin (DNR).

BCX inhibits lamellipodia formation in lung cells.

Cytotoxic activity of HER2-lytic hybrid peptide.

VP16-E2 is a more potent transcriptional activator than Gal4-VP16.

Gramicidin A (GA) decreases HIF protein expression in RCC cells.

Synergistic effect of CNP and doxorubicin (DOX) on thiol oxidation.

PX-478 directly radiosensitizes tumor cells in vitro.

IL1R8 deficiency is associated with increased susceptibility to lymphoma development in lpr mice. IL1R8 deficiency is associated with increased susceptibility.

Effect of HA on hematopoietic recovery in tumor-bearing mice.

Peripheral CAF model. Peripheral CAF model. A, bright field (top) and fluorescence (bottom) micrographs of aggregates that contain RFP-tagged 344SQ cells.

PTENP1 sensitizes renal cancer cells to chemotherapy.

EGCG inhibits cell survival and growth of EFT cell lines but not of HBMECs. A, dose-responsive curves of fractional survival of EFT cells (TC32 and TC71)

Identification of NSC as a FADD-kinase inhibitor.

A, chemical structures of PRIMA-1 and PRIMA-1Met.

CCG regulates cellular localization and blocks target gene expression of MRTF. A, SK-Mel-147 cells were cotransfected with RhoC and the SRE.L reporter.

Effects of WDL on in vitro invasion and soft agar colony formation by LNCaP cells. Effects of WDL on in vitro invasion and soft agar colony formation by.

TSA-mediated changes in cell cycle inhibitor proteins.

Selective delivery of pIC/PPHAffibody decreases the survival of HER2-overexpressing cells. Selective delivery of pIC/PPHAffibody decreases the survival.

FOXO1 inhibits Runx2-induced invasion of prostate cancer cells.

The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.

Effect of G9a on the expression of GSH synthesis enzymes.

Extracellular matrix and type I collagen promote a scattered phenotype and induce cell motility. Extracellular matrix and type I collagen promote a scattered.

Drug sensitivities of IGROV1 cells to AGF94 and PMX at leucovorin (LCV) concentrations of 2 and 25 nmol/L. Drug sensitivities of IGROV1 cells to AGF94.

Cisplatin plus PTUPB decreases proliferation and angiogenesis but increases apoptosis as determined by immunohistochemical (IHC) analysis. Cisplatin plus.

HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential.

Src expression in a panel of human TCC cell lines.

Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

Colony formation of K-562 cells after 24 h (upper panel) or 48 h (lower panel) exposure to ErPC3 (20 μm) and ASO-bcr (10 μm), respectively. Colony formation.

Effect of miRNA mimic on platinum sensitivity.

Inhibition of EGFR kinase activity and autophosphorylation by Iressa.

Targeting HR via CDK inhibition resensitizes recurrent cultures to temozolomide (TMZ). Targeting HR via CDK inhibition resensitizes recurrent cultures.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Xenograft regrowth studies.

Inhibitory effect of SFN pretreatment on the TPA-induced transformation of shMock- and shNrf2-transfected JB6 P+ cells. Inhibitory effect of SFN pretreatment.

Inhibitory effects of SFN on the TPA-induced transformation of JB6 P+ cells. Inhibitory effects of SFN on the TPA-induced transformation of JB6 P+ cells.

Endogenously produced n-3 PUFAs inhibit endometrial cancer cell growth in vitro and in vivo. Endogenously produced n-3 PUFAs inhibit endometrial cancer.

Increased migration and podia formation in NFIB-suppressed human osteosarcoma cells. Increased migration and podia formation in NFIB-suppressed human osteosarcoma.

Incorporation of the TSTA system into a single plasmid.

Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. LNCaP,

The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. The effect of different concentrations of WMC-79.

CLIC1 supports invadopodia formation in vitro and metastasis in vivo.

Effects of EGCG on the anchorage-independent growth of EFT cells.

Pdcd4 expression inhibits AP-1 transactivation.

Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. A,

BMX inhibition suppresses the growth of CRPC cells in vitro and in vivo. BMX inhibition suppresses the growth of CRPC cells in vitro and in vivo. A, CWR22RV1.

BEZ235 induces accelerated senescence after radiation (IR) in vitro.

ABT-888 sensitizes multiple ovarian cancer cell lines but not normal cells to FdUrd. ABT-888 sensitizes multiple ovarian cancer cell lines but not normal.

Detection of protein levels of Cdc25AWT and its mutation derivatives in breast cancer cells with I3C treatment. Detection of protein levels of Cdc25AWT.

BME treatment increases cytotoxic activity of NK3

T3 or T4 induces MAPK activation in myeloma cells.

Determination of O2− production by H&E staining using confocal fluorescence microscope. Determination of O2− production by H&E staining using confocal.

DHA and dietary n-3 PUFAs inhibit endometrial cancer cell growth in vitro and in xenograft models. DHA and dietary n-3 PUFAs inhibit endometrial cancer.

Presentation transcript:

UNC569 reduces colony formation in Jurkat and 697 cell lines. UNC569 reduces colony formation in Jurkat and 697 cell lines. Cells were plated in methylcellulose in the presence of the indicated concentrations of UNC569 or DMSO control only. Every 48 hours, fresh culture medium–containing UNC569 or DMSO was added. Colonies were counted after 8 days. Long-term colony growth of 697 (A) and Jurkat (B) cells in methylcellulose is shown. Mean values ± SEs were derived from three independent experiments. *, P ≤ 0.05. C, representative colonies from the Jurkat and 697 cell lines after treatment with the indicated concentrations of UNC569 or DMSO control only. Single colony pictures were generated using a Nikon Eclipse TS100 microscope with Nikon Plan 10X, numerical aperture 0.25 objective lens, and with a Nikon Digital Sight DS-L2 camera equipped with Firmware DS-L2 Ver4.60. D, representative fields from methylcellulose cultures of the Jurkat and 697 cell lines after treatment with UNC569 or DMSO control only. Bright field images were taken with a spinning disk confocal 3I Marianas based on an inverted Zeiss Axio Observer Z1. A CoolSnap HQ2 camera featuring 1,392 × 1,040 imaging pixels via a 5× Zeiss air objective was used. As imaging acquisition software Slidebook 5.0 was used. Sandra Christoph et al. Mol Cancer Ther 2013;12:2367-2377 ©2013 by American Association for Cancer Research