Functional Expression of C-Terminally Modified AMT1;3 Variants in the Quadruple Insertion Line qko.(A) Influx of 15N-labeled ammonium into roots of qko.

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Functional Expression of C-Terminally Modified AMT1;3 Variants in the Quadruple Insertion Line qko.(A) Influx of 15N-labeled ammonium into roots of qko and two independent lines of qko-35S:AMT1;3, qko-35S:AMT1;3TA, or qko-35S:AMT1;3TD. Functional Expression of C-Terminally Modified AMT1;3 Variants in the Quadruple Insertion Line qko.(A) Influx of 15N-labeled ammonium into roots of qko and two independent lines of qko-35S:AMT1;3, qko-35S:AMT1;3TA, or qko-35S:AMT1;3TD. Six-week-old Arabidopsis plants were precultured in nutrient solution containing 2 mM ammonium nitrate and subjected to N deficiency for a period of 4 d prior to influx analysis.15N-labeled ammonium was supplied at a concentration of 200 μM. Bars indicate means ± sd; n = 8 to 10. Significant differences at P < 0.01 according to Tukey’s test are indicated by different letters. DW, dry weight.(B) Protein gel blot analysis of microsomal membrane fractions from roots of qko and one independent line of qko-35S:AMT1;3, qko-35S:AMT1;3TA, or qko-35S:AMT1;3TD using the anti-AMT1;3 antibody. Protein samples were pretreated either at 37°C in the presence of β-mercaptoethanol (reducing conditions; +βME) or at 0°C in the absence of β-mercaptoethanol (nonreducing conditions; −βME). MM, Magic Marker.[See online article for color version of this figure.] Lixing Yuan et al. Plant Cell 2013;25:974-984 ©2013 by American Society of Plant Biologists